Please use this identifier to cite or link to this item: https://doi.org/10.1039/c0lc00221f
Title: Mechanics of cell spreading within 3D-micropatterned environments
Authors: Ghibaudo, M.
Di Meglio, J.-M.
Hersen, P. 
Ladoux, B. 
Issue Date: 7-Mar-2011
Citation: Ghibaudo, M., Di Meglio, J.-M., Hersen, P., Ladoux, B. (2011-03-07). Mechanics of cell spreading within 3D-micropatterned environments. Lab on a Chip - Miniaturisation for Chemistry and Biology 11 (5) : 805-812. ScholarBank@NUS Repository. https://doi.org/10.1039/c0lc00221f
Abstract: Most tissue cells evolve in vivo in a three-dimensional (3D) microenvironment including complex topographical patterns. Cells exert contractile forces to adhere and migrate through the extracellular matrix (ECM). Although cell mechanics has been extensively studied on 2D surfaces, there are too few approaches that give access to the traction forces that are exerted in 3D environments. Here, we describe an approach to measure dynamically the contractile forces exerted by fibroblasts while they spread within arrays of large flexible micropillars coated with ECM proteins. Contrary to very dense arrays of microposts, the density of the micropillars has been chosen to promote cell adhesion in between the pillars. Cells progressively impale onto the micropatterned substrate. They first adhere on the top of the pillars without applying any detectable forces. Then, they spread along the pillar sides, spanning between the elastic micropillars and applying large forces on the substrate. Interestingly, the architecture of the actin cytoskeleton and the adhesion complexes vary over time as cells pull on the pillars. In particular, we observed less stress fibers than for cells spread on flat surfaces. However, prominent actin stress fibers are observed at cell edges surrounding the micropillars. They generate increasing contractile forces during cell spreading. Cells treated with blebbistatin, a myosin II inhibitor, relax their internal tension, as observed by the release of pillar deformations. Moreover, cell spreading on pillars coated with ECM proteins only on their tops are not able to generate significant traction forces. Taken together, these findings highlight the dynamic relationship between cellular forces and acto-myosin contractility in 3D environments, the influence of cytoskeletal network mechanics on cell shape, as well as the importance of cell-ECM contact area in the generation of traction forces. © 2011 The Royal Society of Chemistry.
Source Title: Lab on a Chip - Miniaturisation for Chemistry and Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/126672
ISSN: 14730197
DOI: 10.1039/c0lc00221f
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

70
checked on Jan 19, 2022

WEB OF SCIENCETM
Citations

64
checked on Jan 19, 2022

Page view(s)

93
checked on Jan 20, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.