Please use this identifier to cite or link to this item:
DC FieldValue
dc.titleMonovalent H5 vaccine based on epitope-chimeric HA provides broad cross-clade protection against variant H5N1 viruses in mice
dc.contributor.authorHe, F.
dc.contributor.authorPrabakaran, M.
dc.contributor.authorKumar, S.R.
dc.contributor.authorTan, Y.
dc.contributor.authorKwang, J.
dc.identifier.citationHe, F., Prabakaran, M., Kumar, S.R., Tan, Y., Kwang, J. (2014). Monovalent H5 vaccine based on epitope-chimeric HA provides broad cross-clade protection against variant H5N1 viruses in mice. Antiviral Research 105 (1) : 143-151. ScholarBank@NUS Repository.
dc.description.abstractH5N1 HPAI virus continues to be a severe threat for public health, as well as for the poultry industry, due to its high mortality and antigenic drift rate. There is no monovalent vaccine available which provides broad protection against those major circulating strains. In the present study, a monovalent H5 vaccine strain was developed with antigenic sequence analysis and epitope mutations. H5 from Indonesia strain (A/Indonesia/CDC669/2006) was used as backbone sequence. Three amino acids were mutated to express immunogenic epitopes from other circulating H5N1s in the backbone. RG influenza virus expressing the epitope-chimeric H5 can react in HI with multiple H5 monoclonal antibodies which fail to neutralize wild type CDC669. High titers in HI and virus neutralization against different clades H5N1s (clade 1, 2, 4 and 7) were detected using sera from mice immunized with the epitope-chimeric H5N1. The monovalent vaccine with RG-epitope-chimeric H5N1 protected mice from lethal challenge with H5N1s of different clades, including clade 1.0, 2.1, 2.2 and 2.3. This study indicates that the broad immune response elicited by this single H5N1 virus allows it to be a promising candidate for a monovalent H5 universal vaccine. © 2014 Elsevier B.V. All rights reserved.
dc.subjectCross clade
dc.subjectH5N1 AIV
dc.subjectInfluenza vaccine
dc.description.sourcetitleAntiviral Research
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.