Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.antiviral.2014.03.002
DC FieldValue
dc.titleMonovalent H5 vaccine based on epitope-chimeric HA provides broad cross-clade protection against variant H5N1 viruses in mice
dc.contributor.authorHe, F.
dc.contributor.authorPrabakaran, M.
dc.contributor.authorKumar, S.R.
dc.contributor.authorTan, Y.
dc.contributor.authorKwang, J.
dc.date.accessioned2016-07-08T09:28:00Z
dc.date.available2016-07-08T09:28:00Z
dc.date.issued2014
dc.identifier.citationHe, F., Prabakaran, M., Kumar, S.R., Tan, Y., Kwang, J. (2014). Monovalent H5 vaccine based on epitope-chimeric HA provides broad cross-clade protection against variant H5N1 viruses in mice. Antiviral Research 105 (1) : 143-151. ScholarBank@NUS Repository. https://doi.org/10.1016/j.antiviral.2014.03.002
dc.identifier.issn18729096
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/125507
dc.description.abstractH5N1 HPAI virus continues to be a severe threat for public health, as well as for the poultry industry, due to its high mortality and antigenic drift rate. There is no monovalent vaccine available which provides broad protection against those major circulating strains. In the present study, a monovalent H5 vaccine strain was developed with antigenic sequence analysis and epitope mutations. H5 from Indonesia strain (A/Indonesia/CDC669/2006) was used as backbone sequence. Three amino acids were mutated to express immunogenic epitopes from other circulating H5N1s in the backbone. RG influenza virus expressing the epitope-chimeric H5 can react in HI with multiple H5 monoclonal antibodies which fail to neutralize wild type CDC669. High titers in HI and virus neutralization against different clades H5N1s (clade 1, 2, 4 and 7) were detected using sera from mice immunized with the epitope-chimeric H5N1. The monovalent vaccine with RG-epitope-chimeric H5N1 protected mice from lethal challenge with H5N1s of different clades, including clade 1.0, 2.1, 2.2 and 2.3. This study indicates that the broad immune response elicited by this single H5N1 virus allows it to be a promising candidate for a monovalent H5 universal vaccine. © 2014 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.antiviral.2014.03.002
dc.sourceScopus
dc.subjectCross clade
dc.subjectH5N1 AIV
dc.subjectInfluenza vaccine
dc.subjectMonovalent
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.antiviral.2014.03.002
dc.description.sourcetitleAntiviral Research
dc.description.volume105
dc.description.issue1
dc.description.page143-151
dc.description.codenARSRD
dc.identifier.isiut000335631100020
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

11
checked on Jan 27, 2022

WEB OF SCIENCETM
Citations

10
checked on Jan 27, 2022

Page view(s)

91
checked on Jan 27, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.