Please use this identifier to cite or link to this item:
Title: Chikungunya virus envelope-specific human monoclonal antibodies with broad neutralization potency
Authors: Warter, L.
Lee, C.Y.
Thiagarajan, R.
Grandadam, M.
Lebecque, S.
Lin, R.T.P. 
Bertin-Maghit, S.
Ng, L.F.P.
Abastado, J.-P.
Desprès, P.
Wang, C.-I.
Nardin, A.
Issue Date: 1-Mar-2011
Citation: Warter, L., Lee, C.Y., Thiagarajan, R., Grandadam, M., Lebecque, S., Lin, R.T.P., Bertin-Maghit, S., Ng, L.F.P., Abastado, J.-P., Desprès, P., Wang, C.-I., Nardin, A. (2011-03-01). Chikungunya virus envelope-specific human monoclonal antibodies with broad neutralization potency. Journal of Immunology 186 (5) : 3258-3264. ScholarBank@NUS Repository.
Abstract: Chikungunya virus (CHIKV) is an alphavirus responsible for numerous epidemics in Africa and Asia. Infection by CHIKV is often characterized by long-lasting, incapacitating arthritis, and some fatal cases have been described among elderly and newborns. Currently, there is no available vaccine or specific treatment against CHIKV. Blood B cells from a donor with history of CHIKV infection were activated, immortalized, amplified, and cloned. Two human mAbs against CHIKV, 5F10 and 8B10, were identified, sequenced, and expressed in recombinant form for characterization. In a plaque reduction neutralization test, 5F10 and 8B10 show mean IC50 of 72 and 46 ng/ml, respectively. Moreover, both mAbs lead to a strong decrease in extracellular spreading of infectious viral particles from infected to uninfected cells. Importantly, the mAbs neutralize different CHIKV isolates from Singapore, Africa, and Indonesia, as well as O'nyong-nyong virus, but do not recognize other alphaviruses tested. Both mAbs are specific for the CHIKV envelope: 5F10 binds to the E2 glycoprotein ectodomain and 8B10 to E1 and/or E2. In conclusion, these two unique human mAbs strongly, broadly, and specifically neutralize CHIKV infection in vitro and might become possible therapeutic tools against CHIKV infection, especially in individuals at risk for severe disease. Importantly, these mAbs will also represent precious tools for future studies on host - pathogen interactions and the rational design of vaccines against CHIKV. Copyright © 2011 by The American Association of Immunologists, Inc.
Source Title: Journal of Immunology
ISSN: 00221767
DOI: 10.4049/jimmunol.1003139
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jul 7, 2020


checked on Jul 7, 2020

Page view(s)

checked on Jun 26, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.