Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jviromet.2010.04.001
Title: A simple method for Alexa Fluor dye labelling of dengue virus
Authors: Zhang, S.L.X.
Tan, H.-C. 
Hanson, B.J.
Ooi, E.E. 
Keywords: Alexa Fluor
Dengue virus
Fluorescence
Fluorescent virus
Labelling
Issue Date: Aug-2010
Citation: Zhang, S.L.X., Tan, H.-C., Hanson, B.J., Ooi, E.E. (2010-08). A simple method for Alexa Fluor dye labelling of dengue virus. Journal of Virological Methods 167 (2) : 172-177. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jviromet.2010.04.001
Abstract: Dengue virus causes frequent and cyclical epidemics throughout the tropics, resulting in significant morbidity and mortality rates. There is neither a specific antiviral treatment nor a vaccine to prevent epidemic transmission. The lack of a detailed understanding of the pathogenesis of the disease complicates these efforts. The development of methods to probe the interaction between the virus and host cells would thus be useful. Direct fluorescence labelling of virus would facilitate the visualization of the early events in virus-cell interaction. This report describes a simple method of labelling of dengue virus with Alexa Fluor succinimidyl ester dye dissolved directly in the sodium bicarbonate buffer that yielded highly viable virus after labelling. Alexa Fluor dyes have superior photostability and are less pH-sensitive than the common dyes, such as fluorescein and rhodamine, making them ideal for studies on cellular uptake and endosomal transport of the virus. The conjugation of Alexa Fluor dye did not affect the recognition of labelled dengue virus by virus-specific antibody and its putative receptors in host cells. This method could have useful applications in virological studies. © 2010 Elsevier B.V.
Source Title: Journal of Virological Methods
URI: http://scholarbank.nus.edu.sg/handle/10635/125300
ISSN: 01660934
DOI: 10.1016/j.jviromet.2010.04.001
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