Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-61779-319-6_13
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dc.titleNanostructured TiO 2 thin films for phosphoproteomics studies with MALDI mass spectrometry
dc.contributor.authorTorta, F.
dc.contributor.authorFusi, M.
dc.contributor.authorCasari, C.S.
dc.contributor.authorBassi, A.L.
dc.contributor.authorBachi, A.
dc.date.accessioned2014-12-12T08:01:22Z
dc.date.available2014-12-12T08:01:22Z
dc.date.issued2011
dc.identifier.citationTorta, F.,Fusi, M.,Casari, C.S.,Bassi, A.L.,Bachi, A. (2011). Nanostructured TiO 2 thin films for phosphoproteomics studies with MALDI mass spectrometry. Methods in Molecular Biology 790 : 173-181. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/978-1-61779-319-6_13" target="_blank">https://doi.org/10.1007/978-1-61779-319-6_13</a>
dc.identifier.isbn9781617793189
dc.identifier.issn10643745
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/117087
dc.description.abstractAlterations in protein phosphorylation, a posttranslational modification (PTM) that regulates many-processes in living cells, is a fundamental mechanism of many diseases, including cancer. Phosphoproteomics, with the combined use of affinity chromatography and electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, is shedding light into phosphorylation signaling pathways at the proteome level and helps to solve difficulties related to sample complexity and phosphopeptide enrichment. One of the most frequent and efficient methods used to enrich samples for the phosphorylated components is titanium dioxide chromatography. Titanium dioxide has a high affinity for phosphopeptides and can also be selective in specific experimental conditions. Here, we describe a protocol for the use of a MALDI plate covered with titanium dioxide nanostructured film, a device developed for a rapid and efficient study of phosphorylated peptides. © 2011 Springer Science+Business Media, LLC.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/978-1-61779-319-6_13
dc.sourceScopus
dc.subjectMALDI
dc.subjectMass spectrometry
dc.subjectPhosphoproteomics
dc.subjectPulsed laser deposition
dc.subjectTitanium dioxide
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.description.doi10.1007/978-1-61779-319-6_13
dc.description.sourcetitleMethods in Molecular Biology
dc.description.volume790
dc.description.page173-181
dc.identifier.isiutNOT_IN_WOS
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