Please use this identifier to cite or link to this item: https://doi.org/10.1089/ten.tea.2011.0315
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dc.titleImproved mesenchymal stem cells attachment and in vitro cartilage tissue formation on chitosan-modified poly(l-lactide-co-epsilon-caprolactone) scaffold
dc.contributor.authorYang, Z.
dc.contributor.authorWu, Y.
dc.contributor.authorLi, C.
dc.contributor.authorZhang, T.
dc.contributor.authorZou, Y.
dc.contributor.authorHui, J.H.P.
dc.contributor.authorGe, Z.
dc.contributor.authorLee, E.H.
dc.date.accessioned2014-12-12T08:00:50Z
dc.date.available2014-12-12T08:00:50Z
dc.date.issued2012-02-01
dc.identifier.citationYang, Z., Wu, Y., Li, C., Zhang, T., Zou, Y., Hui, J.H.P., Ge, Z., Lee, E.H. (2012-02-01). Improved mesenchymal stem cells attachment and in vitro cartilage tissue formation on chitosan-modified poly(l-lactide-co-epsilon-caprolactone) scaffold. Tissue Engineering - Part A 18 (3-4) : 242-251. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.tea.2011.0315
dc.identifier.issn19373341
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/117046
dc.description.abstractConsidering the load-bearing physiological requirement of articular cartilage, scaffold for cartilage tissue engineering should exhibit appropriate mechanical responses as natural cartilage undergoing temporary deformation on loading with little structural collapse, and recovering to the original geometry on unloading. A porous elastomeric poly l-lactide-co-ε-caprolactone (PLCL) was generated and crosslinked at the surface to chitosan to improve its wettability. Human bone marrow derived mesenchymal stem cells (MSC) attachment, morphological change, proliferation and in vitro cartilage tissue formation on the chitosan-modified PLCL scaffold were compared with the unmodified PLCL scaffold. Chitosan surface promoted more consistent and even distribution of the seeded MSC within the scaffold. MSC rapidly adopted a distinct spread-up morphology on attachment on the chitosan-modified PLCL scaffold with the formation of F-actin stress fiber which proceeded to cell aggregation; an event much delayed in the unmodified PLCL. Enhanced cartilage formation on the chitosan-modified PLCL was shown by real-time PCR analysis, histological and immunochemistry staining and biochemical assays of the cartilage extracellular matrix components. The Young's modulus of the derived cartilage tissues on the chitosan-modified PLCL scaffold was significantly increased and doubled that of the unmodified PLCL. Our results show that chitosan modification of the PLCL scaffold improved the cell compatibility of the PLCL scaffold without significant alteration of the physical elastomeric properties of PLCL and resulted in the formation of cartilage tissue of better quality. © 2012 Mary Ann Liebert, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1089/ten.tea.2011.0315
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.description.doi10.1089/ten.tea.2011.0315
dc.description.sourcetitleTissue Engineering - Part A
dc.description.volume18
dc.description.issue3-4
dc.description.page242-251
dc.identifier.isiut000300003300003
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