Analysis of the roles of pins and heterotrimeric G proteins in asymmetric division of Drosophila neuroblasts

Abstract

Drosophila neuroblasts divide asymmetrically to give rise to two daughter cells of distinct fates and sizes. A number of studies in asymmetric cell division have begun to elucidate the molecular mechanisms underlying asymmetric proteinRNA localization, spindle orientation, and spindle asymmetry. This article describes methods of analyzing the role of Pins and heterotrimeric G proteins in Drosophila neuroblast asymmetric division.