Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/116574
DC Field | Value | |
---|---|---|
dc.title | Reporter enzyme assays. | |
dc.contributor.author | Pardy, K. | |
dc.date.accessioned | 2014-12-12T07:51:32Z | |
dc.date.available | 2014-12-12T07:51:32Z | |
dc.date.issued | 1993 | |
dc.identifier.citation | Pardy, K. (1993). Reporter enzyme assays.. Methods in molecular biology (Clifton, N.J.) 18 : 419-424. ScholarBank@NUS Repository. | |
dc.identifier.issn | 19406029 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/116574 | |
dc.description.abstract | Reporter genes code for proteins that have a unique enzymatic activity and are used to assess the transcriptional properties of DNA elements. The use of reporter genes in transgenic animals provides a rapid method for the detection of transgene expression, which is easily distinguishable from expression of the corresponding endogenous gene of the animal. The regulatory sequences of a chosen gene are fused to a readily assayable protein coding region, examples of which are chloramphenicol acetyl transferase (CAT), β-galactosidase, and luciferase. Sensitive assays are available for each of these proteins that facilitate detection and quantitation of transgene expression. The use of these reporter enzymes allows a more rapid and sensitive method of detection than the analysis of specific transgene transcripts within the transgenic animals. The reporter enzymes described in this chapter are CAT, β-galactosidase, and luciferase. | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.sourcetitle | Methods in molecular biology (Clifton, N.J.) | |
dc.description.volume | 18 | |
dc.description.page | 419-424 | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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