Please use this identifier to cite or link to this item:
|Title:||Identification and molecular characterization of a naturally occurring RNA virus mutant defective in the initiation of host recovery||Authors:||Xin, H.-W.
|Issue Date:||20-Dec-2003||Citation:||Xin, H.-W., Ding, S.-W. (2003-12-20). Identification and molecular characterization of a naturally occurring RNA virus mutant defective in the initiation of host recovery. Virology 317 (2) : 253-262. ScholarBank@NUS Repository. https://doi.org/10.1016/j.virol.2003.08.009||Abstract:||The host recovery response is characterized by the disappearance of disease symptoms and activation of the RNA silencing virus resistance in the new growth following an initial symptomatic infection. However, it is not clear what triggers the initiation of recovery, which occurs naturally only in some virus-host interactions. Here we report the identification and characterization of a spontaneous mutant of Tobacco streak virus (TSV) that became defective in triggering recovery in tobacco plants. Infectious full-length cDNA clones corresponding to the tripartite RNA genome were constructed from both the wild-type and the nonrecovery mutant of TSV (TSVnr), the first sets of infectious cDNA clones from an Ilarvirus. Genetic and molecular analyses identified an A → G mutation in the TSVnr genome that was sufficient to confer nonrecovery when introduced into TSV. The mutation was located in the intergenic region of RNA 3 upstream of the mapped transcriptional start site of the coat protein mRNA. Intriguingly, induction of recovery by TSV was not accompanied by virus clearance and TSV consistently accumulated to significantly higher levels than TSVnr did even though TSVnr-infected plants displayed severe symptoms throughout the course of infection. Thus, our findings indicate that recovery of host can be initiated by minimal genetic changes in a viral genome and may occur in the absence of virus clearance. Mechanisms possibly involved in the initiation of host recovery are discussed. © 2003 Elsevier Inc. All rights reserved.||Source Title:||Virology||URI:||http://scholarbank.nus.edu.sg/handle/10635/116398||ISSN:||00426822||DOI:||10.1016/j.virol.2003.08.009|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 18, 2019
WEB OF SCIENCETM
checked on Nov 18, 2019
checked on Nov 8, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.