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|Title:||Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans||Authors:||Myers, C.D.
|Issue Date:||Mar-1996||Citation:||Myers, C.D., Goh, P.-Y., Allen, T.StC., Bucher, E.A., Bogaert, T. (1996-03). Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans. Journal of Cell Biology 132 (6) : 1061-1077. ScholarBank@NUS Repository. https://doi.org/10.1083/jcb.132.6.1061||Abstract:||We have been investigating a set of genes, collectively called mups, that are essential to striated body wall muscle cell positioning in Caenorhabditis elegans. Here we report our detailed characterization of the mup-2 locus, which encodes troponin T (TnT). Mutants for a heat-sensitive allele, called mup-2(e2346ts), and for a putative null, called mup-2(up1), are defective for embryonic body wall muscle cell contraction, sarcomere organization, and cell positioning. Characterizations of the heat-sensitive allele demonstrate that mutants are also defective for regulated muscle contraction in larval and adult body wall muscle, defective for function of the nonstriated oviduct myoepithelial sheath, and defective for epidermal morphogenesis. We cloned the mup-2 locus and its corresponding cDNA. The cDNA encodes a predicted 405- amino acid protein homologous to vertebrate and invertebrate TnT and includes an invertebrate-specific COOH-terminal tail. The mup-2 mutations lie within these cDNA sequences: mup-2(up1) is a termination codon near NH2 terminus (Glu94) and mup-2(e2346ts) is a termination codon in the COOH-terminal invertebrate-specific tail (Trp342). TnT is a muscle contractile protein that, in association with the thin filament proteins tropomyosin, troponin I and troponin C, regulates myosin-actin interaction in response to a rise in intracellular Ca2+. Our findings demonstrate multiple essential functions for TnT and provide a basis to investigate the in vivo functions and protein interactions of TnT in striated and nonstriated muscles.||Source Title:||Journal of Cell Biology||URI:||http://scholarbank.nus.edu.sg/handle/10635/116291||ISSN:||00219525||DOI:||10.1083/jcb.132.6.1061|
|Appears in Collections:||Staff Publications|
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