Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.93.15.7470
Title: An interspecies hybrid RNA virus is significantly more virulent than either parental virus
Authors: Ding, S.-W. 
Shi, B.-J.
Li, W.-X.
Symons, R.H.
Keywords: cucumber mosaic virus
hybrid virus
plant RNA viruses
virus movement
virus synergism
Issue Date: 23-Jul-1996
Citation: Ding, S.-W., Shi, B.-J., Li, W.-X., Symons, R.H. (1996-07-23). An interspecies hybrid RNA virus is significantly more virulent than either parental virus. Proceedings of the National Academy of Sciences of the United States of America 93 (15) : 7470-7474. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.93.15.7470
Abstract: Cucumber mosaic cucumovirus (CMV) infects a very wide range of plant species (> 1000 species). We recently demonstrated that a previously undescribed gene (2b) encoded by RNA 2 of the tripartite RNA genome of CMV is required for systemic virus spread and disease induction in its hosts. Herein we report that when this CMV gene is replaced by its homologue from tomato aspermy cucumovirus (TAV), the resultant hybrid virus is significantly more virulent, induces earlier onset of systemic symptoms, and accumulates to a higher level in seven host species from three families than either of the parents. Our results indicate that CMV and the TAV 2b protein interact synergistically despite the fact that no synergism occurs in double infections with the two parental viruses. To our knowledge, this is the first example of an interspecific hybrid made from plant or animal RNA viruses that is more efficient in systemic infection of a number of hosts than the naturally occurring parents. As CMV and the hybrid virus accumulated to a similar level in the infected tobacco protoplasts, the observed synergistic responses most likely resulted from an increased efficacy of the hybrid virus in systemic spread in host plants provided by the TAV 2b protein. The relevance of our finding to the application of pathogen-derived resistance is discussed.
Source Title: Proceedings of the National Academy of Sciences of the United States of America
URI: http://scholarbank.nus.edu.sg/handle/10635/116222
ISSN: 00278424
DOI: 10.1073/pnas.93.15.7470
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