Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/115905
DC Field | Value | |
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dc.title | Regulation of mRNA 1 expression by the 5'-untranslated region (5'-UTR) of the coronavirus infectious bronchitis virus (IBV) | |
dc.contributor.author | Liu, D.X. | |
dc.contributor.author | Xu, H.Y. | |
dc.contributor.author | Lim, K.P. | |
dc.date.accessioned | 2014-12-12T07:33:57Z | |
dc.date.available | 2014-12-12T07:33:57Z | |
dc.date.issued | 1998 | |
dc.identifier.citation | Liu, D.X.,Xu, H.Y.,Lim, K.P. (1998). Regulation of mRNA 1 expression by the 5'-untranslated region (5'-UTR) of the coronavirus infectious bronchitis virus (IBV). Advances in Experimental Medicine and Biology 440 : 303-311. ScholarBank@NUS Repository. | |
dc.identifier.issn | 00652598 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/115905 | |
dc.description.abstract | In this report, we show that expression of the coronavirus IBV mRNA1 is regulated by its 5'-UTR. Evidence presented demonstrates that the IBV sequence from nucleotide 1 to 1904 directs very inefficient synthesis of a product of approximately 43 kDa. Deletion of either the first 362 bp or the whole part of the 5'-UTR, however, dramatically increased the expression of the 43 kDa protein species. The mechanisms involved were investigated by two different approaches. Firstly, translation of the same construct in the presence of [3H]-leucine ruled out the possibility that initiation of small reading frames from non-AUG codons located in the 5'-UTR may compete with the authentic AUG initiation codon, and therefore inhibit the expression of ORF 1a. Secondly, expression and deletion analyses of a dicistronic construct showed that translation of the 43 kDa protein was initiated by ribosome internal entry mechanism. These studies suggest that a 'weak' ribosome internal entry signal is located in the 5'-UTR and is involved in the regulation of mRNA1 expression. | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR AGROBIOLOGY | |
dc.description.sourcetitle | Advances in Experimental Medicine and Biology | |
dc.description.volume | 440 | |
dc.description.page | 303-311 | |
dc.description.coden | AEMBA | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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