Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/115899
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dc.titleRapid changes in poly (A) tail length of vasopressin and oxytocin mRNAs form a common early component of neurohypophyseal peptide gene activation following physiological stimulation
dc.contributor.authorCarter, D.A.
dc.contributor.authorMurphy, D.
dc.date.accessioned2014-12-12T07:33:53Z
dc.date.available2014-12-12T07:33:53Z
dc.date.issued1991
dc.identifier.citationCarter, D.A.,Murphy, D. (1991). Rapid changes in poly (A) tail length of vasopressin and oxytocin mRNAs form a common early component of neurohypophyseal peptide gene activation following physiological stimulation. Neuroendocrinology 53 (1) : 1-6. ScholarBank@NUS Repository.
dc.identifier.issn00283835
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/115899
dc.description.abstractThe time course of acute changes in vasopressin (VP) and oxytocin (OT) mRNA size and level during dehydration has been studied in rats. Total RNA was extracted from samples of the supraoptic nucleus at various intervals after water deprivation, subjected to northern blotting, and probed with oligonucleotides specific for VP and OT mRNA. The VP and OT mRNA size, shown previously to reflect 3'-poly (A) tail length, was consistently increased 2 h after dehydration, prior to significant changes in plasma osmolality or haematocrit. Intraperitoneal administration of hypertonic saline resulted in a similarly rapid VP and OT mRNA size response, in some cases within 1 h of treatment. The effect of a discrete hypovolaemic stimulus was investigated with intraperitoneal injections of polyethylene glycol; again, the VP and OT mRNA size was rapidly increased. No significant changes in mRNA level were observed in any of the experimental groups. The results show that an increase in VP and OT mRNA poly (A) tail length forms an acute and general response to activation of the hypothalamo-neurohypophyseal system. The rapidity of the poly (A) tail response, which appears to be independent of physiological signalling mechanisms associated with increases in mRNA accumulation (observed after 2 days of dehydration), provides a paradigm for the investigation of novel modes of neuronal gene regulation.
dc.sourceScopus
dc.subjecthypovolaemia
dc.subjectmRNA
dc.subjectosmolality
dc.subjectoxytocin
dc.subjectpoly(A)tail
dc.subjectsupraoptic nucleus
dc.subjectvasopressin
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.sourcetitleNeuroendocrinology
dc.description.volume53
dc.description.issue1
dc.description.page1-6
dc.description.codenNUNDA
dc.identifier.isiutNOT_IN_WOS
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