Please use this identifier to cite or link to this item: https://doi.org/10.1093/pcp/pci226
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dc.titleLarge-scale systematic study on stability of the Ds element and timing of transposition in rice
dc.contributor.authorSzeverenyi, I.
dc.contributor.authorRamamoorthy, R.
dc.contributor.authorZhi, W.T.
dc.contributor.authorHong, F.L.
dc.contributor.authorZhi, G.M.
dc.contributor.authorRamachandran, S.
dc.date.accessioned2014-12-12T07:32:31Z
dc.date.available2014-12-12T07:32:31Z
dc.date.issued2006-01
dc.identifier.citationSzeverenyi, I., Ramamoorthy, R., Zhi, W.T., Hong, F.L., Zhi, G.M., Ramachandran, S. (2006-01). Large-scale systematic study on stability of the Ds element and timing of transposition in rice. Plant and Cell Physiology 47 (1) : 84-95. ScholarBank@NUS Repository. https://doi.org/10.1093/pcp/pci226
dc.identifier.issn00320781
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/115790
dc.description.abstractActivator/Dissociation (Ac/Ds) transposon mutagenesis is a widely used tool for gene identification; however, several reports on silencing of the Ac/Ds element in starter lines and in stable transposants question the applicability of such an approach in later generations. We have performed a systematic analysis on various aspects of the silencing phenomenon in rice (Oryza sativa ssp. japonica cv. Nipponbare). High somatic and germinal transposition frequencies observed in earlier generations were maintained as late as T4 and T5 generations; thus the propagation of parental lines did not induce transposon silencing. Moreover, the stably transposed Ds element was active even at the F5 generation, since Ac could remobilize the Ds element as indicated by the footprint analysis of several revertants. Expression of the bar gene was monitored from F3 to F6 generations in >1,000 lines. Strikingly, substantial transgene silencing was not observed in any of the generations tested. We analyzed the timing of transposition during rice development and provide evidence that Ds is transposed late after tiller formation. The possibility, that the independent events could be the result of secondary transposition, was ruled out by analyzing potential footprints by reciprocal PCR. Our study validates the Ac/Ds system as a tool for large-scale mutagenesis in rice, since the Ds elements were active in the starter and insertion lines even in the later generations. We propose that harvesting rice seeds using their panicles is an alternative way to increase the number of independent transposants due to post-tillering transposition. JSPP © 2006.
dc.sourceScopus
dc.subjectAc/Ds transposon
dc.subjectIndependent transposition
dc.subjectRice
dc.subjectSilencing
dc.subjectTiming
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR AGROBIOLOGY
dc.description.doi10.1093/pcp/pci226
dc.description.sourcetitlePlant and Cell Physiology
dc.description.volume47
dc.description.issue1
dc.description.page84-95
dc.description.codenPCPHA
dc.identifier.isiut000235218800009
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