Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/115777
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dc.titleInteraction of oligonucleotides containing 6-O-methylguanine with human DNA (cytosine-5-)-methyltransferase
dc.contributor.authorTan, N.-W.
dc.contributor.authorLi, B.F.L.
dc.date.accessioned2014-12-12T07:32:21Z
dc.date.available2014-12-12T07:32:21Z
dc.date.issued1990
dc.identifier.citationTan, N.-W.,Li, B.F.L. (1990). Interaction of oligonucleotides containing 6-O-methylguanine with human DNA (cytosine-5-)-methyltransferase. Biochemistry® 29 (39) : 9234-9240. ScholarBank@NUS Repository.
dc.identifier.issn00062960
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/115777
dc.description.abstractThirty-base-pair synthetic oligonucleotide duplexes containing a single meG·C (meG = 6-O-methylguanine) or A·C base pair at the 16th position (i.e., 5′-CCCGTTTAAATATACATATACCCGGGTACC-3′, where X = A or meG) were used to study de novo methylation by the purified human DNA (cytosine-5)-methyltransferase isolated from CEM cells. Both duplexes containing meG-C and A·C base pairs show enhanced methyl group acceptor properties. Subsequent introduction of hemimethylated sites at the 15th position of the top strand (the C residue next to the abnormal base pair) and the 7th, 15th (which represents the C residue in the 6meG·C and A·C base pairs), and 27th positions of the bottom strand were used to study the maintenance methylation of the hemimethylated duplexes by the methylase. This revealed striking differences in the rate, amount, and sites of methylation, which are dependent on the position of the hemimethylated site in the duplex. The possible mechanism of action of the methylase is discussed. The data show that 6-Omethylguanine residues in DNA can have other genetic effects apart from their miscoding behavior and that meG·C and A·C base pairs exert different effects in terms of methylation. © 1990 American Chemical Society.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.sourcetitleBiochemistry®
dc.description.volume29
dc.description.issue39
dc.description.page9234-9240
dc.identifier.isiutNOT_IN_WOS
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