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Title: Hormonal influence on amylase gene expression during Seabass (Lates calcarifer) larval development
Authors: Ma, P.
Reddy, K.P. 
Chan, W.K. 
Lam, T.J. 
Keywords: α-Amylase
Issue Date: Aug-2004
Citation: Ma, P., Reddy, K.P., Chan, W.K., Lam, T.J. (2004-08). Hormonal influence on amylase gene expression during Seabass (Lates calcarifer) larval development. General and Comparative Endocrinology 138 (1) : 14-19. ScholarBank@NUS Repository.
Abstract: α-Amylase gene expression was detected in newly hatched seabass (Lates calcarifer) larvae and peaked at around first feeding. This suggests a greater importance of carbohydrates during early larval development than might be expected for carnivorous fishes. In vivo cortisol and triiodothyronine (T3) treatment of seabass larvae upregulated α-amylase gene expression. The identification of a functional glucocorticoid-response element (GRE) on the amylase gene promoter indicates that cortisol (glucocorticoid) stimulation of amylase gene expression is direct via GRE. However no TRE (thyroid-response element) was found on the amylase gene and its promoter, and various concentrations of T3 (1nM-10μM) also did not induce α-amylase gene promoter activity in rat AR42-J cells transfected with the promoter construct, unlike dexamethasone treatment. This suggests that T 3 stimulation of amylase gene expression in vivo was indirect, probably secondary to its promotion of one or more developmental processes. © 2004 Elsevier Inc. All rights reserved.
Source Title: General and Comparative Endocrinology
ISSN: 00166480
DOI: 10.1016/j.ygcen.2004.04.007
Appears in Collections:Staff Publications

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