Please use this identifier to cite or link to this item: https://doi.org/10.2741/3113
Title: DNA-directed assembly of protein microarrays
Authors: Tang, Y.C.
Wan, G. 
Ng, J.K.
Ajikumar, P.K.
Too, H.-P.
Keywords: Analyte-specific reagents
DNA protein conjugates
DNA-directed assembly
Microarray
Protein array
Review
Self-assembly
Issue Date: 1-May-2008
Citation: Tang, Y.C.,Wan, G.,Ng, J.K.,Ajikumar, P.K.,Too, H.-P. (2008-05-01). DNA-directed assembly of protein microarrays. Frontiers in Bioscience 13 (15) : 5755-5771. ScholarBank@NUS Repository. https://doi.org/10.2741/3113
Abstract: Microarray technology has made it possible to simultaneously study the abundance, interactions, and functions of potentially tens of thousands of biological molecules. From its earliest use in DNA microarrays, where only nucleic acids were captured and detected on the arrays, applications of microarrays now extend to those involving biomolecules such as antibodies, proteins, peptides, and carbohydrates. In contrast to the relative robustness of DNA microarrays, the use of such chemically diverse biomolecules on microarray formats presents many challenges in their fabrication as well as application. Among the many methods that have been proposed to overcome these challenges, DNA-directed assembly (DDA) has emerged as a promising strategy for the high sensitivity and multiplexed capture and detection of various analytes. In this review, we explore the challenges faced during the design, fabrication, and utilization of protein microarrays and highlight how DDA strategies, together with other recent advances in the field, are accelerating the development of platforms available for protein microarray applications.
Source Title: Frontiers in Bioscience
URI: http://scholarbank.nus.edu.sg/handle/10635/114665
ISSN: 10939946
DOI: 10.2741/3113
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