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Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-62703-733-4_1
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dc.titleHigh-throughput synchronization of mammalian cell cultures by spiral microfluidics
dc.contributor.authorLee, W.C.
dc.contributor.authorBhagat, A.A.S.
dc.contributor.authorLim, C.T.
dc.date.accessioned2014-12-02T08:05:34Z
dc.date.available2014-12-02T08:05:34Z
dc.date.issued2014
dc.identifier.citationLee, W.C.,Bhagat, A.A.S.,Lim, C.T. (2014). High-throughput synchronization of mammalian cell cultures by spiral microfluidics. Methods in Molecular Biology 1104 : 3-13. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/978-1-62703-733-4_1" target="_blank">https://doi.org/10.1007/978-1-62703-733-4_1</a>
dc.identifier.isbn9781627037327
dc.identifier.issn10643745
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/114511
dc.description.abstractThe development of mammalian cell cycle synchronization techniques has greatly advanced our understanding of many cellular regulatory events and mechanisms specific to different phases of the cell cycle. In this chapter, we describe a high-throughput microfluidic-based approach for cell cycle synchronization. By exploiting the relationship between cell size and its phase in the cell cycle, large numbers of synchronized cells can be obtained by size fractionation in a spiral microfluidic channel. Protocols for the synchronization of primary cells such as mesenchymal stem cells, and immortal cell lines such as Chinese hamster ovarian cells (CHO-CD36) and HeLa cells are provided as examples. © 2014 Springer Science+Business Media, LLC.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/978-1-62703-733-4_1
dc.sourceScopus
dc.subjectCancer cells
dc.subjectCell cycle synchronization
dc.subjectCell separation
dc.subjectMicrofluidics
dc.subjectStem cells
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1007/978-1-62703-733-4_1
dc.description.sourcetitleMethods in Molecular Biology
dc.description.volume1104
dc.description.page3-13
dc.identifier.isiutNOT_IN_WOS
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