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https://doi.org/10.1016/S0014-5793(03)00213-8
DC Field | Value | |
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dc.title | ArhGAP15, a novel human RacGAP protein with GTPase binding property | |
dc.contributor.author | Seoh, M.L. | |
dc.contributor.author | Ng, C.H. | |
dc.contributor.author | Yong, J. | |
dc.contributor.author | Lim, L. | |
dc.contributor.author | Leung, T. | |
dc.date.accessioned | 2014-12-01T06:57:39Z | |
dc.date.available | 2014-12-01T06:57:39Z | |
dc.date.issued | 2003-03-27 | |
dc.identifier.citation | Seoh, M.L., Ng, C.H., Yong, J., Lim, L., Leung, T. (2003-03-27). ArhGAP15, a novel human RacGAP protein with GTPase binding property. FEBS Letters 539 (1-3) : 131-137. ScholarBank@NUS Repository. https://doi.org/10.1016/S0014-5793(03)00213-8 | |
dc.identifier.issn | 00145793 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/113718 | |
dc.description.abstract | We have previously described a partial cDNA sequence encoding a RhoGAP protein, GAP25 that is homologous to the recently reported ArhGAP9 and ArhGAP12. We now describe a related new member ArhGAP15 that shares a number of domain similarities, including a pleckstrin homology (PH) domain, a RhoGAP domain and a novel motif N-terminal to the GAP domain. This novel motif was found to be responsible for nucleotide-independent Rac1 binding. Using swop mutants of Rac/Cdc42, we have established that the binding is through the C-terminal half of Rac1. The GAP domain of ArhGAP15 showed specificity towards Rac1 in vitro. The PH domain is required for ArhGAP15 to localize to cell periphery and over-expression of the full-length ArhGAP15, but not the mutant with a partial deletion of the PH domain, resulted in an increase in actin stress fibers and cell contraction. These morphological effects can be attenuated by the co-expression of dominant negative Rac1N17. HeLa cells expressing ArhGAP15 were also resistant to phorbol myristatate acetate treatment, suggesting that ArhGAP15 is a potential regulator of Rac1. © 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0014-5793(03)00213-8 | |
dc.source | Scopus | |
dc.subject | Actin cytoskeleton | |
dc.subject | GAP | |
dc.subject | Rac1 | |
dc.subject | Rac1-binding | |
dc.type | Conference Paper | |
dc.contributor.department | ANATOMY | |
dc.description.doi | 10.1016/S0014-5793(03)00213-8 | |
dc.description.sourcetitle | FEBS Letters | |
dc.description.volume | 539 | |
dc.description.issue | 1-3 | |
dc.description.page | 131-137 | |
dc.description.coden | FEBLA | |
dc.identifier.isiut | 000181957400026 | |
Appears in Collections: | Staff Publications |
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