Please use this identifier to cite or link to this item: https://doi.org/10.1158/0008-5472.CAN-04-3875
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dc.titleProtein kinase C inhibition and X-linked inhibitor of apoptosis protein degradation contribute to the sensitization effect of luteolin on tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in cancer cells
dc.contributor.authorShi, R.-X.
dc.contributor.authorOng, C.-N.
dc.contributor.authorShen, H.-M.
dc.date.accessioned2014-12-01T06:56:28Z
dc.date.available2014-12-01T06:56:28Z
dc.date.issued2005-09-01
dc.identifier.citationShi, R.-X., Ong, C.-N., Shen, H.-M. (2005-09-01). Protein kinase C inhibition and X-linked inhibitor of apoptosis protein degradation contribute to the sensitization effect of luteolin on tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in cancer cells. Cancer Research 65 (17) : 7815-7823. ScholarBank@NUS Repository. https://doi.org/10.1158/0008-5472.CAN-04-3875
dc.identifier.issn00085472
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/113614
dc.description.abstractTumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is an important member of the TNF superfamily with great potential in cancer therapy. Luteolin is a dietary flavonoid commonly found in some medicinal plants. Here we found that pretreatment with a noncytotoxic concentration of luteolin significantly sensitized TRAIL-induced apoptosis in both TRAIL-sensitive (HeLa) and TRAIL-resistant cancer cells (CNE1, HT29, and HepG2). Such sensitization is achieved through enhanced caspase-8 activation and caspase-3 maturation. Further, the protein level of X-linked inhibitor of apoptosis protein (XIAP) was markedly reduced in cells treated with luteolin and TRAIL, and ectopic expression of XIAP protected against cell death induced by luteolin and TRAIL, showing that luteolin sensitizes TRAIL-induced apoptosis through down-regulation of XIAP. In search of the molecular mechanism responsible for XIAP down-regulation, we found that luteolin and TRAIL promoted XIAP ubiquitination and proteasomal degradation. Next, we showed that protein kinase C (PKC) activation prevented cell death induced by luteolin and TRAIL via suppression of XIAP down-regulation. Moreover, luteolin inhibited PKC activity, and bisindolylmaleimide I, a general PKC inhibitor, simulated luteolin in sensitizing TRAIL-induced apoptosis. Taken together, these results present a novel anticancer effect of luteolin and support its potential application in cancer therapy in combination with TRAIL. In addition, our data reveal a new function of PKC in cell death: PKC activation stabilizes XIAP and thus suppresses TRAIL-induced apoptosis. ©2005 American Association for Cancer Research.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1158/0008-5472.CAN-04-3875
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCOMMUNITY,OCCUPATIONAL & FAMILY MEDICINE
dc.description.doi10.1158/0008-5472.CAN-04-3875
dc.description.sourcetitleCancer Research
dc.description.volume65
dc.description.issue17
dc.description.page7815-7823
dc.description.codenCNREA
dc.identifier.isiut000231659500039
Appears in Collections:Staff Publications

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