Please use this identifier to cite or link to this item: https://doi.org/10.1006/enrs.1999.4025
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dc.titleInvolvement of oxidative stress in crystalline silica-induced cytotoxicity and genotoxicity in rat alveolar macrophages
dc.contributor.authorZhang, Z.
dc.contributor.authorShen, H.-M.
dc.contributor.authorZhang, Q.-F.
dc.contributor.authorOng, C.-N.
dc.date.accessioned2014-12-01T06:55:35Z
dc.date.available2014-12-01T06:55:35Z
dc.date.issued2000-03
dc.identifier.citationZhang, Z., Shen, H.-M., Zhang, Q.-F., Ong, C.-N. (2000-03). Involvement of oxidative stress in crystalline silica-induced cytotoxicity and genotoxicity in rat alveolar macrophages. Environmental Research 82 (3) : 245-252. ScholarBank@NUS Repository. https://doi.org/10.1006/enrs.1999.4025
dc.identifier.issn00139351
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/113535
dc.description.abstractAlveolar macrophages (AMs) occupy a key position in silica-induced pulmonary fibrosis, although the mechanism3 are yet to be elucidated. In the present study we examined the involvement of oxidative stress and reactive oxygen species formation in silica-induced cytotoxicity and genotoxicity in cultured rat AMs. A lucigenin-dependent chemiluminescence test was used to determine superoxide anion (O2/-), and a 2',7'-dichlorofluorescin diacetate fluorescence test was employed to measure the hydrogen peroxide (H2O2) level. The cytotoxic and genotoxic effects caused by silica in AMs were examined by lactate dehydrogenase (LDH) leakage and single-cell gel electrophoresis (comet assay), respectively. The results showed that silica enhanced O2/- and H2O2 formation in AMs. There were clear dose- and time- dependent relationships in silica-induced cytotoxicity and genotoxicity. Furthermore, superoxide dismutase and catalase were able to reduce silica- induced LDH leakage and DNA damage, with concurrent significant inhibition on silica-induced oxidative stress in AMs. These findings provide convincing evidence that oxidative stress mediates the silica-induced cytotoxicity and genotoxicity. The understanding of such a mechanism may provide a scientific basis for the possible application of antioxidants in preventing the hazardous effects of silica. (C) 2000 Academic Press.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1006/enrs.1999.4025
dc.sourceScopus
dc.subjectAlveolar macrophage
dc.subjectCrystalline silica
dc.subjectCytotoxicity
dc.subjectGenotoxicity
dc.subjectOxidative stress
dc.subjectReactive oxygen species
dc.typeArticle
dc.contributor.departmentCOMMUNITY,OCCUPATIONAL & FAMILY MEDICINE
dc.description.doi10.1006/enrs.1999.4025
dc.description.sourcetitleEnvironmental Research
dc.description.volume82
dc.description.issue3
dc.description.page245-252
dc.description.codenENVRA
dc.identifier.isiut000085846300007
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