Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bcp.2004.03.032
DC Field | Value | |
---|---|---|
dc.title | Inhibitory effect of emodin on tumor invasion through suppression of activator protein-1 and nuclear factor-κB | |
dc.contributor.author | Huang, Q. | |
dc.contributor.author | Shen, H.-M. | |
dc.contributor.author | Ong, C.-N. | |
dc.date.accessioned | 2014-12-01T06:55:30Z | |
dc.date.available | 2014-12-01T06:55:30Z | |
dc.date.issued | 2004-07-15 | |
dc.identifier.citation | Huang, Q., Shen, H.-M., Ong, C.-N. (2004-07-15). Inhibitory effect of emodin on tumor invasion through suppression of activator protein-1 and nuclear factor-κB. Biochemical Pharmacology 68 (2) : 361-371. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bcp.2004.03.032 | |
dc.identifier.issn | 00062952 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/113528 | |
dc.description.abstract | 3-Methyl-1,6,8-trihydroxyanthraquinone (emodin) is an active component from the rhizome of Rheum palmatum, a widely used traditional Chinese herb. In this study, we found that emodin significantly inhibited 12-O-tetradecanoylphorbol- 13-acetate (TPA)-induced in vitro invasion of human cancer cells including HSC5 and MDA-MB-231 cells. Matrix metalloproteinases (MMPs) are known to be associated with cancer invasion. Zymographic analysis showed that emodin suppressed TPA-induced MMP-9 activity in a concentration-dependent manner. We further demonstrated that emodin reduced the transcriptional activity of activator protein-1 (AP-1) and nuclear factor kappaB (NF-κB), two important nuclear transcription factors involved in MMP-9 expression. Emodin suppressed the phosphorylation of two mitogen-activated protein kinases, extracellular signal-regulated protein kinase and c-Jun N-terminal kinase, but not p38 kinase, leading to reduced c-Jun phosphorylation and AP-1 DNA-binding. Moreover, emodin inhibited TPA-induced degradation of inhibitor of kappaBα, nuclear translocation of p65, and NF-κB DNA-binding activity. Taken together, these results suggest that emodin inhibits the invasiveness of human cancer cells by suppressing MMP-9 expression through inhibiting AP-1 and NF-κB signaling pathways. © 2004 Elsevier Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bcp.2004.03.032 | |
dc.source | Scopus | |
dc.subject | 12-O-tetradecanoylphorbol-13-acetate | |
dc.subject | activator protein-1 | |
dc.subject | AP-1 | |
dc.subject | dithiothreitol | |
dc.subject | DTT | |
dc.subject | MAPK | |
dc.subject | matrix metalloproteinase | |
dc.subject | MMP | |
dc.subject | NF-κB | |
dc.subject | nuclear factor kappaB | |
dc.subject | PAGE | |
dc.subject | polyacrylamide gel electropheresis | |
dc.subject | SDS | |
dc.subject | sodium dodecyl sulfate | |
dc.subject | TPA | |
dc.type | Article | |
dc.contributor.department | COMMUNITY,OCCUPATIONAL & FAMILY MEDICINE | |
dc.description.doi | 10.1016/j.bcp.2004.03.032 | |
dc.description.sourcetitle | Biochemical Pharmacology | |
dc.description.volume | 68 | |
dc.description.issue | 2 | |
dc.description.page | 361-371 | |
dc.description.coden | BCPCA | |
dc.identifier.isiut | 000222308200017 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
SCOPUSTM
Citations
131
checked on Feb 3, 2023
WEB OF SCIENCETM
Citations
127
checked on Feb 3, 2023
Page view(s)
181
checked on Feb 2, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.