Please use this identifier to cite or link to this item:
Title: Expression and purification of a neuropeptide nocistatin using two related plant viral vectors
Authors: Lim, A.A.L.
Tachibana, S. 
Watanabe, Y.
Wong, S.M. 
Keywords: Coat protein
Odontoglossum ringspot virus
Tobacco mosaic virus
Issue Date: 1-May-2002
Citation: Lim, A.A.L., Tachibana, S., Watanabe, Y., Wong, S.M. (2002-05-01). Expression and purification of a neuropeptide nocistatin using two related plant viral vectors. Gene 289 (1-2) : 69-79. ScholarBank@NUS Repository.
Abstract: Both odontoglossum ringspot virus (ORSV) and tobacco mosaic virus (TMV) were investigated as expression viral vectors for the expression of a neuropeptide nocistatin. Chimeras of ORSV and TMV were constructed by fusion of 17 amino acids of mouse nocistatin (mNST) to the C-terminal of the coat protein (CP) gene via a Factor Xa cleavage linker to yield ORSV-mNST and TMV-mNST. Expression of the mNST peptide was demonstrated by immuno-transmission electron microscopy, western blot, mass spectrometry and radioimmunoassay. Serial passaging of the chimeric viruses revealed loss of mNST from TMV-mNST by the fifth passage. The mNST was maintained in ORSV-mNST throughout six passages. The mNST peptide could be effectively cleaved and purified from chimeric ORSV CP. To our knowledge, this is the first successful attempt in obtaining a complete peptide with no additional amino acid sequence after expression and purification through the use of either ORSV or TMV as vectors. © 2002 Elsevier Science B.V. All rights reserved.
Source Title: Gene
ISSN: 03781119
DOI: 10.1016/S0378-1119(02)00468-7
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jan 22, 2020


checked on Jan 13, 2020

Page view(s)

checked on Jan 18, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.