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Title: Expression and purification of a neuropeptide nocistatin using two related plant viral vectors
Authors: Lim, A.A.L.
Tachibana, S. 
Watanabe, Y.
Wong, S.M. 
Keywords: Coat protein
Odontoglossum ringspot virus
Tobacco mosaic virus
Issue Date: 1-May-2002
Citation: Lim, A.A.L., Tachibana, S., Watanabe, Y., Wong, S.M. (2002-05-01). Expression and purification of a neuropeptide nocistatin using two related plant viral vectors. Gene 289 (1-2) : 69-79. ScholarBank@NUS Repository.
Abstract: Both odontoglossum ringspot virus (ORSV) and tobacco mosaic virus (TMV) were investigated as expression viral vectors for the expression of a neuropeptide nocistatin. Chimeras of ORSV and TMV were constructed by fusion of 17 amino acids of mouse nocistatin (mNST) to the C-terminal of the coat protein (CP) gene via a Factor Xa cleavage linker to yield ORSV-mNST and TMV-mNST. Expression of the mNST peptide was demonstrated by immuno-transmission electron microscopy, western blot, mass spectrometry and radioimmunoassay. Serial passaging of the chimeric viruses revealed loss of mNST from TMV-mNST by the fifth passage. The mNST was maintained in ORSV-mNST throughout six passages. The mNST peptide could be effectively cleaved and purified from chimeric ORSV CP. To our knowledge, this is the first successful attempt in obtaining a complete peptide with no additional amino acid sequence after expression and purification through the use of either ORSV or TMV as vectors. © 2002 Elsevier Science B.V. All rights reserved.
Source Title: Gene
ISSN: 03781119
DOI: 10.1016/S0378-1119(02)00468-7
Appears in Collections:Staff Publications

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