Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/113400
Title: Cloning, sequencing and expression of cdna encoding growth hormone from Indian catfish (Heteropneustes fossilis)
Authors: Anathy, V.
Venugopal, T.
Koteeswaran, R.
Pandian, T.J.
Mathavan, S. 
Keywords: CDNA cloning
Expression in E. coil
Growth hormone
Heteropneustes fossilis
Indian catfish
RT-PCR
Sequencing
Zebrafish
Issue Date: 2001
Citation: Anathy, V.,Venugopal, T.,Koteeswaran, R.,Pandian, T.J.,Mathavan, S. (2001). Cloning, sequencing and expression of cdna encoding growth hormone from Indian catfish (Heteropneustes fossilis). Journal of Biosciences 26 (3) : 315-324. ScholarBank@NUS Repository.
Abstract: A tissue-specific cDNA library was constructed using polyA+ RNA from pituitary glands of the Indian catfish Heteropneustes fossilis (Bloch) and a cDNA clone encoding growth hormone (GH) was isolated. Using polymerase chain reaction (PCR) primers representing the conserved regions of fish GH sequences the 3′ region of catfish GH cDNA (540 bp) was cloned by random amplification of cDNA ends and the clone was used as a probe to isolate recombinant phages carrying the full-length cDNA sequence. The full-length cDNA clone is 1132 bp in length, coding for an open reading frame (ORF) of 603 bp; the reading frame encodes a putative polypeptide of 200 amino acids including the signal sequence of 22 amino acids. The 5′ and 3′ untranslated regions of the cDNA are 58 bp and 456 bp long, respectively. The predicted amino acid sequence of H. fossils GH shared 98% homology with other catfishes. Mature GH protein was efficiently expressed in bacterial and zebrafish systems using appropriate expression vectors. The successful expression of the cloned GH cDNA of catfish confirms the functional viability of the clone.
Source Title: Journal of Biosciences
URI: http://scholarbank.nus.edu.sg/handle/10635/113400
ISSN: 02505991
Appears in Collections:Staff Publications

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