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Title: A semiautomated isolation procedure for selecting low-serum-requiring animal cell population
Authors: Lee, Y.-K.
Yap, P.-K.
Teoh, A.-P. 
Keywords: Animal cell culture
isolation method
serum requirement
Issue Date: Aug-1994
Citation: Lee, Y.-K.,Yap, P.-K.,Teoh, A.-P. (1994-08). A semiautomated isolation procedure for selecting low-serum-requiring animal cell population. Enzyme and Microbial Technology 16 (8) : 726-729. ScholarBank@NUS Repository.
Abstract: Isolation of low-serum-requiring mouse-mouse hybridoma and human hybridoma cell population was done in a simple, gradual, and semiautomated selection system. It involved the addition of medium containing a lower serum level than that of the culture vessel. The rate of addition was determined by the rate of acidification of the culture medium in the vessel. Therefore, the whole population was subjected to a gradual and continuous reduction of serum level. Dilution rate was determined by the genetic potential of individual cells. Thus, the lowest-serum-requiring cells could be isolated within the shortest possible time, without the labor of repeating subculturing. © 1994.
Source Title: Enzyme and Microbial Technology
ISSN: 01410229
Appears in Collections:Staff Publications

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