Please use this identifier to cite or link to this item:
|Title:||Simple liquid chromatographic method for the determination of physostigmine and its metabolite eseroline in rat plasma: Application to a pharmacokinetic study||Authors:||Zhao, B.
|Issue Date:||5-Feb-2003||Citation:||Zhao, B., Moochhala, S.M., Chaw, C.S., Yang, Y.Y. (2003-02-05). Simple liquid chromatographic method for the determination of physostigmine and its metabolite eseroline in rat plasma: Application to a pharmacokinetic study. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 784 (2) : 323-329. ScholarBank@NUS Repository. https://doi.org/10.1016/S1570-0232(02)00817-6||Abstract:||Physostigmine, an anticholinergic drug, and its metabolite eseroline can be quantitated by high-performance liquid chromatography (HPLC) with photodiode-array detection. After addition of the structurally related internal standard (-)-N-methylphysostigmine, rat plasma samples were extracted and cleaned using a Varian Bond Elut C18 column. The methanol-ammonia (98:2) eluate was evaporated to dryness and reconstituted with 0.01 M sodium dihydrogenphosphate (pH 3). Physostigmine and eseroline were separated on an Alltech Ultrasphere Silica column (250×4.6 mm I.D.; particle size 5 μm) at a flow-rate of 1 ml/min, with a mobile phase of 0.01 M sodium dihydrogenphosphate (pH 3)-acetonitrile (85:15). The limits of detection were 10 and 25 ng/ml for physostigmine and eseroline, respectively; the signal-to-noise ratio for this concentration was approximately 3:1. Spiked rat plasma containing 0.1-2.5 μg/ml of physostigmine and eseroline gives good linearity. The average percentage recovery from five spiked plasma samples was 88.0±2.9 and 61.1±5.6% for physostigmine and eseroline, respectively. Within the concentration range 0.1-2.5 μg/ml, the within-day precision was 1.9-8.3 and 3.0-7.7% for physostigmine and eseroline, respectively, and the between-day precision was 4.1-9.3 and 3.7-11% for physostigmine and eseroline, respectively. The method is rapid, simple and reliable, thus it is suitable for pharmacokinetic studies in the rat. © 2002 Elsevier Science B.V. All rights reserved.||Source Title:||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences||URI:||http://scholarbank.nus.edu.sg/handle/10635/112809||ISSN:||15700232||DOI:||10.1016/S1570-0232(02)00817-6|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 26, 2021
WEB OF SCIENCETM
checked on Oct 26, 2021
checked on Oct 14, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.