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|Title:||Simple liquid chromatographic method for the determination of physostigmine and its metabolite eseroline in rat plasma: Application to a pharmacokinetic study||Authors:||Zhao, B.
|Issue Date:||5-Feb-2003||Citation:||Zhao, B., Moochhala, S.M., Chaw, C.S., Yang, Y.Y. (2003-02-05). Simple liquid chromatographic method for the determination of physostigmine and its metabolite eseroline in rat plasma: Application to a pharmacokinetic study. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 784 (2) : 323-329. ScholarBank@NUS Repository. https://doi.org/10.1016/S1570-0232(02)00817-6||Abstract:||Physostigmine, an anticholinergic drug, and its metabolite eseroline can be quantitated by high-performance liquid chromatography (HPLC) with photodiode-array detection. After addition of the structurally related internal standard (-)-N-methylphysostigmine, rat plasma samples were extracted and cleaned using a Varian Bond Elut C18 column. The methanol-ammonia (98:2) eluate was evaporated to dryness and reconstituted with 0.01 M sodium dihydrogenphosphate (pH 3). Physostigmine and eseroline were separated on an Alltech Ultrasphere Silica column (250×4.6 mm I.D.; particle size 5 μm) at a flow-rate of 1 ml/min, with a mobile phase of 0.01 M sodium dihydrogenphosphate (pH 3)-acetonitrile (85:15). The limits of detection were 10 and 25 ng/ml for physostigmine and eseroline, respectively; the signal-to-noise ratio for this concentration was approximately 3:1. Spiked rat plasma containing 0.1-2.5 μg/ml of physostigmine and eseroline gives good linearity. The average percentage recovery from five spiked plasma samples was 88.0±2.9 and 61.1±5.6% for physostigmine and eseroline, respectively. Within the concentration range 0.1-2.5 μg/ml, the within-day precision was 1.9-8.3 and 3.0-7.7% for physostigmine and eseroline, respectively, and the between-day precision was 4.1-9.3 and 3.7-11% for physostigmine and eseroline, respectively. The method is rapid, simple and reliable, thus it is suitable for pharmacokinetic studies in the rat. © 2002 Elsevier Science B.V. All rights reserved.||Source Title:||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences||URI:||http://scholarbank.nus.edu.sg/handle/10635/112809||ISSN:||15700232||DOI:||10.1016/S1570-0232(02)00817-6|
|Appears in Collections:||Staff Publications|
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