Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/112162
DC FieldValue
dc.titleYY1 represses human papillomavirus type 16 transcription by quenching AP-1 activity
dc.contributor.authorO'Connor, M.J.
dc.contributor.authorTan, S.-H.
dc.contributor.authorTan, C.-H.
dc.contributor.authorBernard, H.-U.
dc.date.accessioned2014-11-28T02:53:53Z
dc.date.available2014-11-28T02:53:53Z
dc.date.issued1996-10
dc.identifier.citationO'Connor, M.J.,Tan, S.-H.,Tan, C.-H.,Bernard, H.-U. (1996-10). YY1 represses human papillomavirus type 16 transcription by quenching AP-1 activity. Journal of Virology 70 (10) : 6529-6539. ScholarBank@NUS Repository.
dc.identifier.issn0022538X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/112162
dc.description.abstractYY1 is a multifunctional transcription factor that has been shown to regulate the expression of a number of cellular and vital genes, including the human papillomavirus (HPV) oncogenes E6 and E7. In this study, we have analyzed the YYl-mediated repression of the HPV type 16 (HPV-16) E6-E7 promoter. A systematic analysis to identify YY1 sites present in the HPV-16 long control region showed that of 30 potential YY1 binding motifs, 24 bound purified recombinant YY1 protein, but only 10 of these were able to bind YY1 when nuclear extracts of HeLa cells were used. Of these, only a cluster of five sites, located in the vicinity of an AP-1 motif, were found to be responsible for repressing the HPV-16 P97 proranter. All live sites were required for repression, the mutation of any one site giving rise to a four- to sixfold increase in transcriptional activity. The target for YY1-mediated repression was identified as being a highly conserved AP-1 site, and we propose that AP-1 may represent a common target for YY1 repression. We also provide data demonstrating that YY1 can bind the transcriptional coactivator CREB-binding protein and propose a potentially novel mechanism by which YY1 represses AP-1 activity as a result of this YY1-CREB-binding protein interaction.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.sourcetitleJournal of Virology
dc.description.volume70
dc.description.issue10
dc.description.page6529-6539
dc.description.codenJOVIA
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

78
checked on Aug 18, 2022

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.