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https://scholarbank.nus.edu.sg/handle/10635/112132
DC Field | Value | |
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dc.title | The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment | |
dc.contributor.author | Klumperman, J. | |
dc.contributor.author | Schweizer, A. | |
dc.contributor.author | Clausen, H. | |
dc.contributor.author | Tang, B.L. | |
dc.contributor.author | Hong, W. | |
dc.contributor.author | Oorschot, V. | |
dc.contributor.author | Hauri, H.-P. | |
dc.date.accessioned | 2014-11-28T02:53:33Z | |
dc.date.available | 2014-11-28T02:53:33Z | |
dc.date.issued | 1998-11 | |
dc.identifier.citation | Klumperman, J.,Schweizer, A.,Clausen, H.,Tang, B.L.,Hong, W.,Oorschot, V.,Hauri, H.-P. (1998-11). The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment. Journal of Cell Science 111 (22) : 3411-3425. ScholarBank@NUS Repository. | |
dc.identifier.issn | 00219533 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/112132 | |
dc.description.abstract | To establish recycling routes in the early secretory pathway we have studied the recycling of the ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 in HepG2 cells. Immunofluorescence microscopy showed progressive concentration of ERGIC-53 in the Golgi area at 15°C, Upon rewarming to 37°C ERGIC-53 redistributed into the cell periphery often via tubular processes that largely excluded anterograde transported albumin. Immunogold labeling of cells cultured at 37°C revealed ERGIC-53 predominantly in characteristic β-COP-positive tubulo-vesicular clusters both near the Golgi apparatus and in the cell periphery. Concentration of ERGIC-53 at 15°C resulted from both accumulation of ERGIC-53 in the ERGIC and movement of ERGIC membranes closer to the Golgi apparatus. Upon rewarming to 37°C the labeling of ERGIC-53 in the ERGIC rapidly returned to normal levels whereas ERGIC-53's labeling in the cis-Golgi was unchanged. Temperature manipulations had no effect on the average number of ERGIC-53 clusters. Density gradient centrifugation indicated that the surplus ERGIC-53 accumulating in the ERGIC at 15°C was rapidly transported to the ER upon rewarming. These results suggest that the ERGIC is a dynamic membrane system composed of a constant average number of clusters and that the major recycling pathway of ERGIC-53 bypasses the Golgi apparatus. | |
dc.source | Scopus | |
dc.subject | β-COP | |
dc.subject | ER-Golgi intermediate compartment | |
dc.subject | ERGIC-53 | |
dc.subject | Immunoelectron microscopy | |
dc.subject | Protein recycling | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.sourcetitle | Journal of Cell Science | |
dc.description.volume | 111 | |
dc.description.issue | 22 | |
dc.description.page | 3411-3425 | |
dc.description.coden | JNCSA | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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