Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/112128
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dc.titleThe Pseudomonas putida ML2 plasmid-encoded genes for benzene dioxygenase are unusual in codon usage and low in G + C content
dc.contributor.authorTan, H.-M.
dc.contributor.authorTang, H.-Y.
dc.contributor.authorJoannou, C.L.
dc.contributor.authorAbdel-Wahab, N.H.
dc.contributor.authorMason, J.R.
dc.date.accessioned2014-11-28T02:53:31Z
dc.date.available2014-11-28T02:53:31Z
dc.date.issued1993-08-16
dc.identifier.citationTan, H.-M.,Tang, H.-Y.,Joannou, C.L.,Abdel-Wahab, N.H.,Mason, J.R. (1993-08-16). The Pseudomonas putida ML2 plasmid-encoded genes for benzene dioxygenase are unusual in codon usage and low in G + C content. Gene 130 (1) : 33-39. ScholarBank@NUS Repository.
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/112128
dc.description.abstractBenzene dioxygenase, catalyzing the oxidation of benzene to cis-1,2-dihydroxy-cyclohexa-3,5-diene, comprises four polypeptides that are encoded by plasmid pHMT112 of Pseudomonas putida ML2. In this study, the nucleotide (nt) sequences of four genes encoding this enzyme (bedC1C2BA) were determined, and the amino acid (aa) sequences were deduced. The sequence showed significant homology with the chromosomally encoded benzene dioxygenase and toluene dioxygenase genes (73-77% for nt and 83-99% for aa), but not the plasmid-encoded naphthalene dioxygenase genes (20-26% for nt and 32-36% for aa). A conserved motif (Cys-Xaa-His-15-to-17 aa-Cys-Xaa2-His, where Xaa is any aa), proposed to bind the Rieske-type [2Fe-2S] cluster, was identified in the deduced aa sequence of the iron-sulfur proteins. Three regions were also identified in the flavoprotein which are likely to be involved in FAD and NAD+binding. The gene order of bedC1C2BA is consistent with most ring-hydroxylating dioxygenases isolated from Pseudomonas. However, the G + C content of 47% is in contrast to the high G + C content of the Pseudomonas chromosome (63%) and other Pseudomonas plasmids (57%), and with its unique codon usage preference this suggests that bedC1C2BA originated from a host derived from a different genus. © 1993.
dc.sourceScopus
dc.subjectAromatic catabolism
dc.subjectflavoprotein
dc.subjectgene transfer
dc.subjectnucleotide sequence
dc.subjectRieske iron-sulfur proteins
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.sourcetitleGene
dc.description.volume130
dc.description.issue1
dc.description.page33-39
dc.description.codenGENED
dc.identifier.isiutNOT_IN_WOS
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