Please use this identifier to cite or link to this item:
DC FieldValue
dc.titleS1 nuclease protection mapping.
dc.contributor.authorSmith, D.R.
dc.identifier.citationSmith, D.R. (1993). S1 nuclease protection mapping.. Methods in molecular biology (Clifton, N.J.) 18 : 363-372. ScholarBank@NUS Repository.
dc.description.abstractThe use of S1 nuclease to map the start site of a transcription unit is a well-established technique. Based on the method of Berk and Sharp, it has undergone many refinements over the years. S1 nuclease mapping requires a relatively detailed knowledge of the gene structure and sequence data (or a very good restriction map) of the first exon and several hundred bases of upstream sequence. Although S1 nuclease mapping is mainly used to map transcription start sites accurately (to be described in this chapter), this method can also be used to map intron-exon junctions. Note that in order to identify a transcriptional start site unambiguously, S1 nuclease mapping should be used in conjunction with primer extension (Chapter 44).
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.sourcetitleMethods in molecular biology (Clifton, N.J.)
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Mar 16, 2023

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.