Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00232630
Title: Role of polyamines on de novo shoot morphogenesis from cotyledons of Brassica campestris ssp. pekinensis (Lour) Olsson in vitro
Authors: Chi, G.-L. 
Lin, W.-S. 
Lee, J.E.E. 
Pua, E.-C. 
Keywords: Brassica campestris ssp. pekinensis
Chinese cabbage
ethylene biosynthesis
polyamine biosynthesis
shoot morphogenesis
tissue culture
Issue Date: Mar-1994
Citation: Chi, G.-L.,Lin, W.-S.,Lee, J.E.E.,Pua, E.-C. (1994-03). Role of polyamines on de novo shoot morphogenesis from cotyledons of Brassica campestris ssp. pekinensis (Lour) Olsson in vitro. Plant Cell Reports 13 (6) : 323-329. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00232630
Abstract: The promotive effect of ethylene inhibitors (Els), i.e. AgNO3 and aminoethoxyvinylglycine (AVG) on de novo shoot regeneration from cultured cotyledonary explants of Brassica campestris ssp. pekinensis cv. Shantung in relation to polyamines (PAs) was investigated. The endogenous levels of free putrescine and spermidine in the explant decreased sharply after 1-3 days of culture, whereas endogenous spermine increased, irrespective of the absence or presence of Els. AgNO3 at 30 μM did not affect endogenous PAs during two weeks of culture. In contrast, explants grown on medium containing 5 μM AVG produced higher levels of free putrescine and spermine which increased rapidly after three days and reached a peak at 10 days. An exogenous application of 5 mM putrescine also resulted in a similar surge of endogenous free spermine of the explant. More strikingly, shoot regeneration from explants grown in the presence of 1-20 mM putrescine, 0.1-2.5 mM spermidine, or 0.1-1 mM spermine was enhanced after three weeks of culture. However, exogenous PAs generally did not affect ethylene production, and endogenous levels of 1-aminocyclopropane-1-carboxylate (ACC) synthase activity and ACC of the explant. This study shows the PA requirement for shoot regeneration from cotyledons of B. campestris ssp. pekinensis in vitro, and also indicates that the promotive effect of PAs on regeneration may not be due to an inhibition of ethylene biosynthesis. © 1994 Springer-Verlag.
Source Title: Plant Cell Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/112060
ISSN: 07217714
DOI: 10.1007/BF00232630
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