Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/111996
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dc.titleNuclear factor I and epithelial cell-specific transcription of human papillomavirus type 16
dc.contributor.authorApt, D.
dc.contributor.authorChong, T.
dc.contributor.authorLiu, Y.
dc.contributor.authorBernard, H.-U.
dc.date.accessioned2014-11-28T02:52:00Z
dc.date.available2014-11-28T02:52:00Z
dc.date.issued1993-08
dc.identifier.citationApt, D.,Chong, T.,Liu, Y.,Bernard, H.-U. (1993-08). Nuclear factor I and epithelial cell-specific transcription of human papillomavirus type 16. Journal of Virology 67 (8) : 4455-4463. ScholarBank@NUS Repository.
dc.identifier.issn0022538X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/111996
dc.description.abstractThe transcription of human papillomavirus type 16 (HPV-16) is mediated by the viral enhancer. Epithelial cell-specific activation is achieved by the cooperative interaction of apparently ubiquitous transcription factors. One of them, nuclear factor I (NFI), binds seven sites within the HPV-16 enhancer. Point mutations on enhancer fragments, which retain epithelial cell specificity, verify the functional contribution of NFI. In band shift experiments, the epithelial cell-derived NFI proteins CTF-1, CTF-2, and CTF-3 form a characteristic pattern of heterodimeric complexes which are observed in all epithelial cells tested. Divergence from this pattern in fibroblasts, liver cells, and lymphoid cells correlates with the lack of HPV-16 enhancer activation. The HPV-16 enhancer can be activated by CTF-1 in SL-2 cells, which lack NFI-like proteins. However, exogenous CTF-1 fails to overcome the inactivity of the viral enhancer in fibroblasts. Western immunoblot and supershift analysis shows that exogenously introduced CTF-1 proteins form different heterodimer complexes with the given subset of endogenous NFI proteins in epithelial or fibroblast cells. Polymerase chain reaction analysis and cDNA library screens identified the endogenous fibroblast type NFI as NFI-X, an NFI family member originally cloned from hamster liver cells. The strict correlation between the activation or lack of activation of the HPV-16 enhancer and cell-specific subsets of NFI proteins argues for the pivotal role of NFI binding sites in the epithelial cell-specific function of the viral enhancer.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.sourcetitleJournal of Virology
dc.description.volume67
dc.description.issue8
dc.description.page4455-4463
dc.description.codenJOVIA
dc.identifier.isiutNOT_IN_WOS
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