Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/111917
Title: HLA-DRB1(*)01 subtyping by heteroduplex analysis
Authors: Savage, D.A. 
Wood, N.A.P.
Bidwell, J.L.
Hui, K.M. 
Keywords: DNA heteroduplex generator
DRB1(*)01
Group specific amplification
HLA subtyping
Polymerase chain reaction
Issue Date: 1995
Citation: Savage, D.A.,Wood, N.A.P.,Bidwell, J.L.,Hui, K.M. (1995). HLA-DRB1(*)01 subtyping by heteroduplex analysis. Tissue Antigens 45 (2) : 120-124. ScholarBank@NUS Repository.
Abstract: In this report we describe an alternative method for the identification of the four known HLA-DRB1(*)01 alleles, which is based on the generation of unique heteroduplex patterns between the different DRB1(*)01 alleles and a synthetic DNA heteroduplex generator (DHG) molecule. The method is technically simple, rapid and cost-effective, as it essentially involves only a single polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis. This technique allows the rapid discrimination of the various known HLA-DRB1(*)01 subtypes, both in homozygous and heterozygous situations. We propose that this technology can potentially be applied to most HLA class I and class II subtyping.
Source Title: Tissue Antigens
URI: http://scholarbank.nus.edu.sg/handle/10635/111917
ISSN: 00012815
Appears in Collections:Staff Publications

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