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Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF02821336
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dc.titleA novel method employing polymerase chain reaction to disrupt genes lacking convenient restriction enzyme sites in yeast
dc.contributor.authorWu, M.
dc.contributor.authorShang, H.-S.
dc.contributor.authorYeong, C.-Y.
dc.contributor.authorTan, P.H.-N.
dc.contributor.authorLu, Z.-X.
dc.contributor.authorWong, S.-M.
dc.date.accessioned2014-11-28T02:49:25Z
dc.date.available2014-11-28T02:49:25Z
dc.date.issued1995-02
dc.identifier.citationWu, M., Shang, H.-S., Yeong, C.-Y., Tan, P.H.-N., Lu, Z.-X., Wong, S.-M. (1995-02). A novel method employing polymerase chain reaction to disrupt genes lacking convenient restriction enzyme sites in yeast. Molecular Biotechnology 3 (1) : 72-74. ScholarBank@NUS Repository. https://doi.org/10.1007/BF02821336
dc.identifier.issn10736085
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/111764
dc.description.abstractA novel method employing polymerase chain reaction was developed for the disruption of yeast genes lacking convenient restriction enzyme sites. The method was found to be easy and effective. Using this method, a yeast YKE2 gene (a yeast homolog of murine k-region expressed genes) was successfully disrupted by replacement of HIS3 marker gene. © 1995 Humana Press Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/BF02821336
dc.sourceScopus
dc.subjectgene disruption
dc.subjectPolymerase chain reaction
dc.subjectyeast
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.contributor.departmentBOTANY
dc.description.doi10.1007/BF02821336
dc.description.sourcetitleMolecular Biotechnology
dc.description.volume3
dc.description.issue1
dc.description.page72-74
dc.identifier.isiutA1995QW39600008
Appears in Collections:Staff Publications

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