Toxic and antifeedant effects of allyl disulfide on Tribolium castaneum (Coleoptera: Tenebrionidae) and Sitophilus zeamais (Coleoptera: Curculionidae)

Abstract

Toxicity and antifeedant activity of allyl disulfide, a volatile compound from garlic, Allium sativum L., to adults and larvae of Tribolium castaneum (Herbst) and adults of Sitophilus zeamais Motschulsky were investigated. Topical application was used for contact toxicity, filter paper impregnation for fumigant toxicity, and a flour disk bioassay for antifeedant activity. The filter paper impregnation bioassay also was used to study the effects on egg hatch and the subsequent progeny emergence of T. castaneum. Adult T. castaneum were more susceptible to allyl disulfide than adult S. zeamais in both toxicity tests. Allyl disulfide was 3 times more potent to T. castaneum adults than to S. zeamais in contact toxicity tests, with LD50s of 7.16 and 22.47 μg/mg insect, respectively. In the fumigant bioassay, allyl disulfide was ≃5 times more effective to the adults of T. castaneum than to S. zeamais, with LC50 values of 0.030 and 0.146 μmg/cm2, respectively. The larvae of T. castaneum were more tolerant of allyl disulfide treatment than the adults in both toxicity tests. Egg hatching and subsequent progeny emergence from treated eggs were reduced with increasing concentrations. Allyl disulfide was a more potent antifeedant to T. castaneum adults than either T. castaneum larvae or S. zeamais adults. A concentration of 7.43 mg/g of food deterred feeding by 85.7% among the T. castaneum adults, but feeding was deterred only by 31.5% at 12.77 mg/g of food and 21.2% at 29.15 mg/g of food for T. castaneum larvae and S. zeamais adults, respectively.