Please use this identifier to cite or link to this item: https://doi.org/10.1016/0378-1119(96)00046-7
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dc.titleThe complete sequence of a Singapore isolate of odontoglossum ringspot virus and comparison with other tobamoviruses
dc.contributor.authorChng, C.-G.
dc.contributor.authorWong, S.-M.
dc.contributor.authorMahtani, P.H.
dc.contributor.authorLoh, C.-S.
dc.contributor.authorGoh, C.-J.
dc.contributor.authorKao, M.C.-C.
dc.contributor.authorChung, M.C.-M.
dc.contributor.authorWatanabe, Y.
dc.date.accessioned2014-11-27T07:43:00Z
dc.date.available2014-11-27T07:43:00Z
dc.date.issued1996-06-01
dc.identifier.citationChng, C.-G., Wong, S.-M., Mahtani, P.H., Loh, C.-S., Goh, C.-J., Kao, M.C.-C., Chung, M.C.-M., Watanabe, Y. (1996-06-01). The complete sequence of a Singapore isolate of odontoglossum ringspot virus and comparison with other tobamoviruses. Gene 171 (2) : 155-161. ScholarBank@NUS Repository. https://doi.org/10.1016/0378-1119(96)00046-7
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/111049
dc.description.abstractThe complete sequence of a Singapore isolate of odontoglossum ringspot virus (ORSV-S1) comprises 6609 nucleotides (nt) and four open reading frames (ORFs 1 to 4). The 126/183-kDa RNA-dependent RNA polymerase (RdRp), 33-kDa movement protein (MP) and 18-kDa coat protein (CP) cistrons are located at nt 63-3401/4901, 4807-5718, and 5721-6197 on the genome, respectively. The 5' UTR contains three copies of an 8-base direct repeat and (CAA)(n) motifs. Characteristic tRNA-like structure and three consecutive homologous regions were present in the 3' UTR. The genomic RNA and MP of ORSV-S1 are one of the longest among all members of the TOV group. Phylogenetic analysis of all four genes indicates evolutionary divergence, but within each gene there are some degrees of evolutionary convergence. The conserved amino acid sequences in the MP can be used for the classification of tobamoviruses.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0378-1119(96)00046-7
dc.sourceScopus
dc.subjectAutomated DNA sequencing
dc.subjectORSV
dc.subjectPlant virus
dc.subjectRecombinant DNA
dc.subjectTobamovirus group
dc.typeArticle
dc.contributor.departmentBIOPROCESSING TECHNOLOGY CENTRE
dc.contributor.departmentBOTANY
dc.description.doi10.1016/0378-1119(96)00046-7
dc.description.sourcetitleGene
dc.description.volume171
dc.description.issue2
dc.description.page155-161
dc.description.codenGENED
dc.identifier.isiutA1996UR51800003
Appears in Collections:Staff Publications

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