Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0006-291X(02)00629-0
Title: C-terminus modification of Streptomyces clavuligerus deacetoxycephalosporin C synthase improves catalysis with an expanded substrate specificity
Authors: Chin, H.S. 
Sim, T.S.
Keywords: C-terminus
Deacetoxycephalosporin C synthase
Penicillin analogue
Site-directed mutagenesis
Issue Date: 2002
Citation: Chin, H.S., Sim, T.S. (2002). C-terminus modification of Streptomyces clavuligerus deacetoxycephalosporin C synthase improves catalysis with an expanded substrate specificity. Biochemical and Biophysical Research Communications 295 (1) : 55-61. ScholarBank@NUS Repository. https://doi.org/10.1016/S0006-291X(02)00629-0
Abstract: The biosynthesis of cephalosporins is catalyzed by deacetoxycephalosporin C synthase (DAOCS). Based on computational, biochemical, and structural analyses, it has been proposed that modification of the C-terminus of DAOCS might be a constructive strategy for engineering improvement in enzyme activity. Therefore, five hydrophilic residues namely N301, Y302, N304, R306, and R307 located in proximity to the C-terminus of Streptomyces clavuligerus DAOCS (scDAOCS) were selected and each substituted with a hydrophobic leucine residue. Substitutions at positions 304, 306, and 307 created mutant scDAOCSs with improved efficiencies in penicillin analog conversion up to 397%. And since it has been previously advocated that the C-terminus is crucial for guiding substrate entry, a truncated mutant DAOCS was constructed to assess its involvement. The truncation of the C-terminus at position 310 in the wild-type scDAOCS resulted in reduction of indiscriminate conversion of penicillin analog but this defect was compensated by the replacement of asparagine with leucine at position 304. © 2002 Elsevier Science (USA). All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/110849
ISSN: 0006291X
DOI: 10.1016/S0006-291X(02)00629-0
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