Please use this identifier to cite or link to this item: https://doi.org/10.1002/stem.507
Title: A Runx1 intronic enhancer marks hemogenic endothelial cells and hematopoietic stem cells
Authors: Ng, C.E.L. 
Yokomizo, T. 
Yamashita, N.
Cirovic, B.
Jin, H.
Wen, Z.
Ito, Y.
Osato, M. 
Keywords: AML1
Enhancer element
Hematopoietic stem cells
Hemogenic endothelial cells
Retrovirus integration site
Issue Date: Oct-2010
Citation: Ng, C.E.L., Yokomizo, T., Yamashita, N., Cirovic, B., Jin, H., Wen, Z., Ito, Y., Osato, M. (2010-10). A Runx1 intronic enhancer marks hemogenic endothelial cells and hematopoietic stem cells. Stem Cells 28 (10) : 1869-1881. ScholarBank@NUS Repository. https://doi.org/10.1002/stem.507
Abstract: Runx1 is essential for the generation of hematopoietic stem cells (HSCs) and is frequently mutated in human leukemias. However, the cis-regulatory mechanisms modulating the Runx1 gene expression remain to be elucidated. Herewith, we report the identification of an intronic Runx1 enhancer, Runx1 +24 mouse conserved noncoding element (mCNE), using a combinatorial in silico approach involving comparative genomics and retroviral integration sites mapping. The Runx1 +24 mCNE was found to possess hematopoietic-specific enhancer activity in both zebra-fish and mouse models. Significantly, this enhancer is active specifically in hemogenic endothelial cells (ECs) at sites where the de novo generation of HSCs occurs. The activity of this enhancer is also strictly restricted to HSCs within the hematopoietic compartment of the adult bone marrow. We anticipate that Runx1 +24 mCNE HSC enhancer will serve as a molecular handle for tracing and/or manipulating hemogenic ECs/HSCs behavior in vivo, and consequently become an invaluable tool for research on stem cell and cancer biology. © AlphaMed Press.
Source Title: Stem Cells
URI: http://scholarbank.nus.edu.sg/handle/10635/110719
ISSN: 10665099
DOI: 10.1002/stem.507
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

53
checked on Oct 22, 2020

WEB OF SCIENCETM
Citations

53
checked on Oct 22, 2020

Page view(s)

128
checked on Oct 23, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.