Please use this identifier to cite or link to this item:
Title: RNA interference by nanofiber-based siRNA delivery system
Authors: Cao, H.
Jiang, X.
Chai, C. 
Chew, S.Y.
Keywords: Electrospinning
Nanofibrous scaffolds
Scaffold-mediated transfection
Tissue engineering
Issue Date: Jun-2010
Citation: Cao, H., Jiang, X., Chai, C., Chew, S.Y. (2010-06). RNA interference by nanofiber-based siRNA delivery system. Journal of Controlled Release 144 (2) : 203-212. ScholarBank@NUS Repository.
Abstract: SiRNA delivery has found useful applications particularly as therapeutic agents against genetic diseases. Currently, the delivery of siRNA typically takes the form of nanoparticles. In order to expand the applications of these potent but labile molecules for long-term use required by tissue engineering and regenerative medicine, alternative delivery vehicles are required. This work presents a scaffold-mediated approach to siRNA delivery. By encapsulating siRNA within polycaprolactone (PCL) nanofibers (300-400. nm in diameter) controlled release of intact siRNA could be achieved for at least 28. days under physiological conditions. The successful transfection of HEK 293 cells with GAPDH siRNA released from fibrous scaffolds at day 5, 15 and 30 demonstrated that the encapsulated molecules remained bioactive throughout the period of sustained release, providing silencing efficiency of 61-81% that was comparable to conventional siRNA transfection. Direct seeding of cells on these biofunctional scaffolds, with and without transfection reagent, demonstrated enhanced cellular uptake and efficient GAPDH gene-silencing. This work demonstrates the potential of nanofibrous scaffold-mediated siRNA delivery for long-term gene-silencing applications. The combination of topographical features provided by nanofibrous scaffolds may provide synergistic contact guidance and biochemical signals to mediate and support cellular development in regenerative medicine. © 2010 Elsevier B.V.
Source Title: Journal of Controlled Release
ISSN: 01683659
DOI: 10.1016/j.jconrel.2010.02.003
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jan 18, 2022


checked on Jan 10, 2022

Page view(s)

checked on Jan 20, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.