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Title: New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells
Authors: Chang, T.H.-T.
Kunasegaran, K.
Tarulli, G.A.
De Silva, D.
Voorhoeve, P.M. 
Pietersen, A.M.
Issue Date: 7-Jan-2014
Citation: Chang, T.H.-T., Kunasegaran, K., Tarulli, G.A., De Silva, D., Voorhoeve, P.M., Pietersen, A.M. (2014-01-07). New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells. Breast Cancer Research 16 (1) : -. ScholarBank@NUS Repository.
Abstract: Introduction: Parity-identified mammary epithelial cells (PI-MECs) are an interesting cellular subset because they survive involution and are a presumptive target for transformation by human epidermal growth factor receptor 2 (HER2)/neu in mammary tumors. Depending on the type of assay, PI-MECs have been designated lobule-restricted progenitors or multipotent stem/progenitor cells. PI-MECs were reported to be part of the basal population of mammary epithelium based on flow cytometry. We investigated the cellular identity and lineage potential of PI-MECs in intact mammary glands. Methods: We performed a quantitative and qualitative analysis of the contribution of PI-MECs to mammary epithelial cell lineages in pregnant and involuted mammary glands by immunohistochemistry, fluorescence-activated cells sorting (FACS), and quantitative polymerase chain reaction. PI-MECs were labeled by the activation of Whey Acidic Protein (WAP)-Cre during pregnancy that results in permanent expression of yellow fluorescent protein. Results: After involution, PI-MECs are present exclusively in the luminal layer of mammary ducts. During pregnancy, PI-MECs contribute to the luminal layer but not the basal layer of alveolar lobules. Strikingly, whereas all luminal estrogen receptor (ER)-negative cells in an alveolus can be derived from PI-MECs, the alveolar ER-positive cells are unlabeled and reminiscent of Notch2-traced L cells. Notably, we observed a significant population of unlabeled alveolar progenitors that resemble PI-MECs based on transcriptional and histological analysis. Conclusions: Our demonstration that PI-MECs are luminal cells underscores that not only basal cells display multi-lineage potential in transplantation assays. However, the lineage potential of PI-MECs in unperturbed mammary glands is remarkably restricted to luminal ER-negative cells of the secretory alveolar lineage. The identification of an unlabeled but functionally similar population of luminal alveolar progenitor cells raises the question of whether PI-MECs are a unique population or the result of stochastic labeling. Interestingly, even when all luminal ER-negative cells of an alveolus are PI-MEC-derived, the basal cells and hormone-sensing cells are derived from a different source, indicating that cooperative outgrowth of cells from different lineages is common in alveologenesis. © 2014 Chang et al.; licensee BioMed Central Ltd.
Source Title: Breast Cancer Research
ISSN: 14655411
DOI: 10.1186/bcr3593
Appears in Collections:Staff Publications

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