Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jviromet.2010.06.013
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dc.titleA method for full genome sequencing of all four serotypes of the dengue virus
dc.contributor.authorChristenbury, J.G.
dc.contributor.authorAw, P.P.K.
dc.contributor.authorOng, S.H.
dc.contributor.authorSchreiber, M.J.
dc.contributor.authorChow, A.
dc.contributor.authorGubler, D.J.
dc.contributor.authorVasudevan, S.G.
dc.contributor.authorOoi, E.E.
dc.contributor.authorHibberd, M.L.
dc.date.accessioned2014-11-26T09:03:26Z
dc.date.available2014-11-26T09:03:26Z
dc.date.issued2010-10
dc.identifier.citationChristenbury, J.G., Aw, P.P.K., Ong, S.H., Schreiber, M.J., Chow, A., Gubler, D.J., Vasudevan, S.G., Ooi, E.E., Hibberd, M.L. (2010-10). A method for full genome sequencing of all four serotypes of the dengue virus. Journal of Virological Methods 169 (1) : 202-206. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jviromet.2010.06.013
dc.identifier.issn01660934
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/110473
dc.description.abstractThe availability of whole genome sequencing has contributed to many aspects of dengue research, and its use in dengue virus (DENV) surveillance for early epidemic warning has been proposed. Methods to sequence the genomes of individual dengue serotypes have been described previously, but no single method is known to be applicable for all four serotypes. This report describes a method for sequencing the entire genome of all four DENV serotypes. Using tagged oligonucleotide primers designed for the 3' end, viral RNA was reverse transcribed into a cDNA spanning the entire genome of each of the four serotypes (DENV-1 to -4). This was followed by amplification of the entire cDNA in five overlapping amplicons. A sequence tag was added to the sense primer annealing to the 5' UTR sequence and the antisense primer annealing to the 3' UTR sequence to ensure no terminal nucleotides were omitted during PCR. Sixty-one virus isolates were sequenced: 58 DENV-2, one DENV-1, one DENV-4 and one DENV-3 published previously. The method described could be applied readily for viral biology studies and incorporated into proactive dengue virologic surveillance. © 2010 Elsevier B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jviromet.2010.06.013
dc.sourceScopus
dc.subjectDengue virus
dc.subjectFull genome sequencing
dc.subjectVirologic surveillance
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1016/j.jviromet.2010.06.013
dc.description.sourcetitleJournal of Virological Methods
dc.description.volume169
dc.description.issue1
dc.description.page202-206
dc.description.codenJVMED
dc.identifier.isiut000282398600030
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