Please use this identifier to cite or link to this item: https://doi.org/10.1186/1471-2121-9-40
DC FieldValue
dc.titleViscoelastic behaviour of human mesenchymal stem cells
dc.contributor.authorTan, S.C.W.
dc.contributor.authorPan, W.X.
dc.contributor.authorMa, G.
dc.contributor.authorCai, N.
dc.contributor.authorLeong, K.W.
dc.contributor.authorLiao, K.
dc.date.accessioned2014-11-26T08:31:18Z
dc.date.available2014-11-26T08:31:18Z
dc.date.issued2008-07-22
dc.identifier.citationTan, S.C.W., Pan, W.X., Ma, G., Cai, N., Leong, K.W., Liao, K. (2008-07-22). Viscoelastic behaviour of human mesenchymal stem cells. BMC Cell Biology 9 : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2121-9-40
dc.identifier.issn14712121
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/110343
dc.description.abstractBackground: In this study, we have investigated the viscoelastic behaviour of individual human adult bone marrow-derived mesenchymal stem cells (hMSCs) and the role of F-actin filaments in maintaining these properties, using micropipette aspiration technique together with a standard linear viscoelastic solid model. Results: Under a room temperature of 20°C, the instantaneous and equilibrium Young's modulus, E0 and E∞, were found to be 886 ± 289 Pa and 372 ± 125 Pa, respectively, while the apparent viscosity, μ, was 2710 ± 1630 Pa·s. hMSCs treated with cytochalasin D up to 20 μM at 20°C registered significant drop of up to 84% in stiffness and increase of up to 255% in viscosity. At the physiological temperature of 37°C, E0 and E∞ have decreased by 42-66% whereas μ has increased by 95%, compared to the control. Majority of the hMSCs behave as viscoelastic solid with a rapid initial increase in aspiration length and it gradually levels out with time. Three other types of non-typical viscoelastic behavior of hMSCs were also seen. Conclusion: hMSCs behave as viscoelastic solid. Its viscoelstic behaviour are dependent on the structural integrity of the F-actin filaments and temperature. © 2008 Tan et al; licensee BioMed Central Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1186/1471-2121-9-40
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1186/1471-2121-9-40
dc.description.sourcetitleBMC Cell Biology
dc.description.volume9
dc.description.page-
dc.description.codenBCBMA
dc.identifier.isiut000258230600001
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