Please use this identifier to cite or link to this item: https://doi.org/10.1093/protein/gzs049
DC FieldValue
dc.titleTransplantation of a hydrogen bonding network from West Nile virus protease onto Dengue-2 protease improves catalytic efficiency and sheds light on substrate specificity
dc.contributor.authorDoan, D.N.P.
dc.contributor.authorLi, K.Q.
dc.contributor.authorBasavannacharya, C.
dc.contributor.authorVasudevan, S.G.
dc.contributor.authorMadhusudhan, M.S.
dc.date.accessioned2014-11-26T08:31:10Z
dc.date.available2014-11-26T08:31:10Z
dc.date.issued2012-12
dc.identifier.citationDoan, D.N.P., Li, K.Q., Basavannacharya, C., Vasudevan, S.G., Madhusudhan, M.S. (2012-12). Transplantation of a hydrogen bonding network from West Nile virus protease onto Dengue-2 protease improves catalytic efficiency and sheds light on substrate specificity. Protein Engineering, Design and Selection 25 (12) : 843-850. ScholarBank@NUS Repository. https://doi.org/10.1093/protein/gzs049
dc.identifier.issn17410126
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/110331
dc.description.abstractThe two-component serine protease of flaviviruses such as Dengue virus (DENV) and West Nile virus (WNV) are attractive targets for inhibitor/ therapeutic design. Peptide aldehyde inhibitors that bind to the covalently tethered two-component WNV protease (WNVpro) with 50 inhibitory concentration (IC50) at sub-micromolar concentrations, bind the equivalent DENV-2 protease (DEN2pro) with IC50 of micromolar concentrations at best. Conversely, the protease inhibitor aprotinin binds DEN2pro ∼1000-fold more tightly than WNVpro. To investigate the residues that are crucial for binding specificity differences, a binding-site network of hydrogen bonds was transplanted from WNVpro onto DEN2pro. The transplantations were a combination of single, double and triple mutations involving S79D, S83N and S85Q. The mutant DENV proteases, except those involving S85Q, proved to be more efficient enzymes, as measured by their kinetic parameters. The binding affinities of the mutants to peptide inhibitors however showed only marginal improvement. Protein structure modeling suggests that the negatively charged residue cluster, Glu89-Glu92, of the NS2B cofactor may play an important role in determining substrate/inhibitor-binding specificity. These same residues may also explain why aprotinin binds more tightly to DEN2pro than WNVpro. Our results suggest that structure-based inhibitor design experiments need to explicitly consider/include this C-terminal region whose negative charge is conserved across the four DENV serotypes and also among the flavivirus family of proteases. © 2012 The Authors.
dc.sourceScopus
dc.subjectDengue virus protease
dc.subjecthomology modelling
dc.subjecthydrogen bonding network
dc.subjectprotein engineering
dc.subjectWest Nile virus protease
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1093/protein/gzs049
dc.description.sourcetitleProtein Engineering, Design and Selection
dc.description.volume25
dc.description.issue12
dc.description.page843-850
dc.description.codenPEDSB
dc.identifier.isiut000311670800006
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