Please use this identifier to cite or link to this item: https://doi.org/10.1021/ac401445a
Title: Paper-based microfluidic electrochemical immunodevice integrated with nanobioprobes onto graphene film for ultrasensitive multiplexed detection of cancer biomarkers
Authors: Wu, Y.
Xue, P.
Kang, Y.
Hui, K.M. 
Issue Date: 17-Sep-2013
Citation: Wu, Y., Xue, P., Kang, Y., Hui, K.M. (2013-09-17). Paper-based microfluidic electrochemical immunodevice integrated with nanobioprobes onto graphene film for ultrasensitive multiplexed detection of cancer biomarkers. Analytical Chemistry 85 (18) : 8661-8668. ScholarBank@NUS Repository. https://doi.org/10.1021/ac401445a
Abstract: To the best of our knowledge, this was the first report on the integration of a signal amplification strategy into a microfluidic paper-based electrochemical immunodevice for the multiplexed measurement of cancer biomarkers. Signal amplification was achieved through the use of graphene to modify the immunodevice surface to accelerate the electron transfer and the use of silica nanoparticles as a tracing tag to label the signal antibodies. Accurate, rapid, simple, and inexpensive point-of-care electrochemical immunoassays were demonstrated using a photoresist-patterned microfluidic paper-based analytical device (μPAD). Using the horseradish peroxidase (HRP)-O-phenylenediamine-H2O2 electrochemical detection system, the potential clinical applicability of this immunodevice was demonstrated through its ability to identify four candidate cancer biomarkers in serum samples from cancer patients. The novel signal-amplified strategy proposed in this report greatly enhanced the sensitivity of the detection of cancer biomarkers. In addition, the electrochemical immunodevice exhibited good stability, reproducibility, and accuracy and thus had potential applications in clinical diagnostics. © 2013 American Chemical Society.
Source Title: Analytical Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/110206
ISSN: 00032700
DOI: 10.1021/ac401445a
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

160
checked on May 5, 2021

WEB OF SCIENCETM
Citations

149
checked on May 5, 2021

Page view(s)

67
checked on May 2, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.