Please use this identifier to cite or link to this item: https://doi.org/10.1167/iovs.11-8403
DC FieldValue
dc.titleManual suction versus femtosecond laser trephination for penetrating keratoplasty: Intraocular pressure, endothelial cell damage, incision geometry, and wound healing responses
dc.contributor.authorAngunawela, R.I.
dc.contributor.authorRiau, A.
dc.contributor.authorChaurasia, S.S.
dc.contributor.authorTan, D.T.
dc.contributor.authorMehta, J.S.
dc.date.accessioned2014-11-26T08:29:19Z
dc.date.available2014-11-26T08:29:19Z
dc.date.issued2012-05
dc.identifier.citationAngunawela, R.I., Riau, A., Chaurasia, S.S., Tan, D.T., Mehta, J.S. (2012-05). Manual suction versus femtosecond laser trephination for penetrating keratoplasty: Intraocular pressure, endothelial cell damage, incision geometry, and wound healing responses. Investigative Ophthalmology and Visual Science 53 (6) : 2571-2579. ScholarBank@NUS Repository. https://doi.org/10.1167/iovs.11-8403
dc.identifier.issn01460404
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/110167
dc.description.abstractPurpose. To measure real-time intraocular pressure (IOP) during trephination with a manual suction trephine (MST) and the femtosecond laser (FSL), and to assess endothelial cell damage, incision geometry, and wound healing response with these procedures. Methods. IOP was monitored with an intracameral sensor. Eight rabbits underwent manual suction trephination. Eight rabbits had FSL trephination (FSL-T). Slit lamp photography, confocal microscopy, and anterior segment optical coherence tomography (AS-OCT) were performed at baseline and postoperatively. Animals were sacrificed at 4 hours and 3 days. Tissue was examined with scanning electron microscopy (SEM) and immunohistochemistry for an array of wound-healing markers. Separately, 6 human corneas had MST (3) and FSL-T (3). Incision geometry was imaged with high resolution Optovue AS-OCT. Results. The average IOP during MST and FSL-T was similar (37 mm Hg). There was wider IOP fluctuation during the MST cutting phase (60 mm Hg maximum). There were 1-2 rows of endothelial loss on either side of the incision for FSL-T and 2-5 rows deep for MST. Immune cell responses at 4 hours (CD11b) were comparable, greater apoptosis with FSL-T (TUNEL) occurred at 4 hours, and there was increased keratocyte proliferation at 3 days (Ki67) with FSL-T. There was significantly greater undercutting of the cornea with MST (46.86 degrees versus 16.72 degrees). Conclusions. There is more IOP variation during MST. Average IOP is 37 mm Hg for both techniques. More endothelial damage and undercutting of the cornea occurs with MST. The wound healing response to FSL-T appears greater at 3 days. © 2012 The Association for Research in Vision and Ophthalmology, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1167/iovs.11-8403
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1167/iovs.11-8403
dc.description.sourcetitleInvestigative Ophthalmology and Visual Science
dc.description.volume53
dc.description.issue6
dc.description.page2571-2579
dc.description.codenIOVSD
dc.identifier.isiut000304864600006
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

22
checked on Jan 30, 2023

WEB OF SCIENCETM
Citations

20
checked on Sep 24, 2021

Page view(s)

104
checked on Feb 2, 2023

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.