Please use this identifier to cite or link to this item: https://doi.org/10.1167/iovs.11-7941
DC FieldValue
dc.titleInvolvement of SPARC and MMP-3 in the pathogenesis of human pterygium
dc.contributor.authorSeet, L.-F.
dc.contributor.authorTong, L.
dc.contributor.authorSu, R.
dc.contributor.authorWong, T.T.
dc.date.accessioned2014-11-26T08:29:04Z
dc.date.available2014-11-26T08:29:04Z
dc.date.issued2012-02
dc.identifier.citationSeet, L.-F., Tong, L., Su, R., Wong, T.T. (2012-02). Involvement of SPARC and MMP-3 in the pathogenesis of human pterygium. Investigative Ophthalmology and Visual Science 53 (2) : 587-595. ScholarBank@NUS Repository. https://doi.org/10.1167/iovs.11-7941
dc.identifier.issn01460404
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/110146
dc.description.abstractPURPOSE. To investigate the expression of SPARC and matrix metalloproteinases (MMPs) in normal conjunctiva and pterygium tissues. METHODS. This study involved paired control or uninvolved conjunctiva and pterygium tissue from 21 patients. Quantitative real-time PCR was performed to assess SPARC and MMP mRNA expression, whereas Western blot analysis was performed to assess SPARC protein levels in normal conjunctiva and pterygium tissue. Tissue localization of SPARC, extracellular matrix proteins, and MMPs were determined by immunofluorescence analyses. RESULTS. SPARC transcript and protein levels were upregulated in pterygium compared with normal conjunctiva. Immunofluorescence analyses showed localization of SPARC to the epithelial basement membrane and stroma of normal conjunctiva tissue. Increased SPARC in the pterygium stroma colocalized partially with elevated collagen I, fibronectin, α-SMA, and MMP-3. SPARC and MMP-3 also colocalized in the pterygium epithelium. CONCLUSIONS. SPARC was upregulated in pterygium and may collaborate with increased MMP-3 in some patients to account for many of the phenotypic properties characteristic of pterygium. © 2012 The Association for Research in Vision and Ophthalmology, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1167/iovs.11-7941
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1167/iovs.11-7941
dc.description.sourcetitleInvestigative Ophthalmology and Visual Science
dc.description.volume53
dc.description.issue2
dc.description.page587-595
dc.description.codenIOVSD
dc.identifier.isiut000302788600007
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

35
checked on Jan 30, 2023

WEB OF SCIENCETM
Citations

27
checked on Sep 28, 2021

Page view(s)

97
checked on Feb 2, 2023

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.