Please use this identifier to cite or link to this item:
Title: Inhibition of isoprenylcysteine carboxylmethyltransferase induces autophagic-dependent apoptosis and impairs tumor growth
Authors: Wang, M. 
Hossain, M.S.
Tan, W. 
Coolman, B.
Zhou, J. 
Liu, S.
Casey, P.J. 
Keywords: apoptosis
cancer therapy
isoprenylcysteine carboxylmethyltransferase (Icmt)
Issue Date: 2-Sep-2010
Citation: Wang, M., Hossain, M.S., Tan, W., Coolman, B., Zhou, J., Liu, S., Casey, P.J. (2010-09-02). Inhibition of isoprenylcysteine carboxylmethyltransferase induces autophagic-dependent apoptosis and impairs tumor growth. Oncogene 29 (35) : 4959-4970. ScholarBank@NUS Repository.
Abstract: Inhibition of isoprenylcysteine carboxylmethyltransferase (Icmt), which catalyzes the final step in the post-translational C-terminal processing of prenylated proteins, suppresses tumor cell growth and induces cell death. Icmt inhibition by either a small molecule inhibitor termed as cysmethynil or inhibitory RNA induces marked autophagy leading to cell death. HepG2 cells were used to investigate the function of autophagy in tumor cell death. Suppression of autophagy, either pharmacologically or through knockdown of the autophagy essential proteins, Atg5 or Atg1, inhibits not only cysmethynil-induced autophagy, but also apoptosis in HepG2 cells. The dependence of cysmethynil-induced apoptosis on autophagy was further shown using autophagy-deficient mouse embryonic fibroblast (MEF) cells. Atg5 / MEF cells were found to be resistant to cysmethynil-induced apoptosis, whereas wild-type MEFs showed high sensitivity to apoptosis induction. These data indicate that inhibition of Icmt can elicit cell death through two linked mechanisms, autophagy and apoptosis, and that autophagy can be an active player upstream of apoptosis in cell types capable of apoptotic cell death, such as HepG2 and MEFs. Further, treatment of mice-bearing HepG2-derived tumors with cysmethynil resulted in marked inhibition of tumor growth analysis of tumor tissue from these mice revealed markers consistent with autophagy induction and cell growth arrest. © 2010 Macmillan Publishers Limited All rights reserved.
Source Title: Oncogene
ISSN: 09509232
DOI: 10.1038/onc.2010.247
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.