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Title: Depletion of macrophages in mice results in higher dengue virus titers and highlights the role of macrophages for virus control
Authors: Fink, K.
Ng, C.
Nkenfou, C.
Vasudevan, S.G. 
Van Rooijen, N.
Schul, W.
Keywords: Dengue virus
Host/pathogen interactions
Issue Date: Oct-2009
Citation: Fink, K., Ng, C., Nkenfou, C., Vasudevan, S.G., Van Rooijen, N., Schul, W. (2009-10). Depletion of macrophages in mice results in higher dengue virus titers and highlights the role of macrophages for virus control. European Journal of Immunology 39 (10) : 2809-2821. ScholarBank@NUS Repository.
Abstract: Monocytes and macrophages are target cells for dengue infection. Besides their potential role for virus replication, activated monocytes/macrophages produce cytokines that may be critical for dengue pathology. To study the in vivo role of monocytes and macrophages for virus replication, we depleted monocytes and macrophages in IFN-αβγR knockout mice with clodronate liposomes before dengue infection. Although less virus was first recovered in the draining LN in the absence of macrophages, monocyte/macrophage depletion eventually resulted in a ten-fold higher systemic viral titer. A massive infiltration of CD11b+CD11clowLy6Clow monocytes into infected organs was observed in parallel with increasing virus titers before viremia was controlled. Depletion of monocytes in the blood before or after local infection had no impact on virus titers, suggesting that monocytes are not required as "virus-shuttles". Our data provide evidence that systemic viremia is established independently of tissue macrophages present at the site of infection and blood monocytes. Instead, we demonstrate the importance of monocytes/macrophages for the control of dengue virus. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
Source Title: European Journal of Immunology
ISSN: 00142980
DOI: 10.1002/eji.200939389
Appears in Collections:Staff Publications

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