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Title: Subcellular fractionation methods and strategies for proteomics
Authors: Lee, Y.H.
Tan, H.T. 
Chung, M.C.M.
Keywords: Cell biology
Subcellular fractionation
Issue Date: Nov-2010
Citation: Lee, Y.H., Tan, H.T., Chung, M.C.M. (2010-11). Subcellular fractionation methods and strategies for proteomics. Proteomics 10 (22) : 3935-3956. ScholarBank@NUS Repository.
Abstract: Developments in subcellular fractionation strategies have provided the means to profile and analyze the protein composition of organelles and cellular structures by proteomics. Here, we review the application of classical (e.g. density gradient centrifugation) and emerging sophisticated techniques (fluorescent-assisted organelle sorting) in the fractionation, and statistical/bioinformatics tools for the prediction of protein localization in subcellular proteomics. We also review the validation methods currently used (such as microscopy, RNA interference and multiple reaction monitoring) and discuss the importance of verification of the results obtained in subcellular proteomics. Finally, the numerous challenges facing subcellular proteomics including the dynamics of organelles are being examined. However, complementary approaches such as modern statistics, bioinformatics and large-scale integrative analysis are beginning to emerge as powerful tools to proteomics for analyzing subcellular organelles and structures. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Source Title: Proteomics
ISSN: 16159853
DOI: 10.1002/pmic.201000289
Appears in Collections:Staff Publications

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