Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2013.07.022
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dc.titleThe Kunitz-protease inhibitor domain in amyloid precursor protein reduces cellular mitochondrial enzymes expression and function
dc.contributor.authorChua, L.-M.
dc.contributor.authorLim, M.-L.
dc.contributor.authorWong, B.-S.
dc.date.accessioned2014-11-26T07:48:54Z
dc.date.available2014-11-26T07:48:54Z
dc.date.issued2013-08-09
dc.identifier.citationChua, L.-M., Lim, M.-L., Wong, B.-S. (2013-08-09). The Kunitz-protease inhibitor domain in amyloid precursor protein reduces cellular mitochondrial enzymes expression and function. Biochemical and Biophysical Research Communications 437 (4) : 642-647. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2013.07.022
dc.identifier.issn0006291X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/109696
dc.description.abstractMitochondrial dysfunction is a prominent feature of Alzheimer's disease (AD) and this can be contributed by aberrant metabolic enzyme function. But, the mechanism causing this enzymatic impairment is unclear. Amyloid precursor protein (APP) is known to be alternatively spliced to produce three major isoforms in the brain (APP695, APP751, APP770). Both APP770 and APP751 contain the Kunitz Protease Inhibitory (KPI) domain, but the former also contain an extra OX-2 domain. APP695 on the other hand, lacks both domains. In AD, up-regulation of the KPI-containing APP isoforms has been reported. But the functional contribution of this elevation is unclear. In the present study, we have expressed and compared the effect of the non-KPI containing APP695 and the KPI-containing APP751 on mitochondrial function. We found that the KPI-containing APP751 significantly decreased the expression of three major mitochondrial metabolic enzymes; citrate synthase, succinate dehydrogenase and cytochrome c oxidase (COX IV). This reduction lowers the NAD+/NADH ratio, COX IV activity and mitochondrial membrane potential. Overall, this study demonstrated that up-regulation of the KPI-containing APP isoforms is likely to contribute to the impairment of metabolic enzymes and mitochondrial function in AD. © 2013 Elsevier Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2013.07.022
dc.sourceScopus
dc.subjectAPP
dc.subjectKPI
dc.subjectMetabolic enzymes
dc.subjectMitochondrial function
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.description.doi10.1016/j.bbrc.2013.07.022
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume437
dc.description.issue4
dc.description.page642-647
dc.description.codenBBRCA
dc.identifier.isiut000323584400025
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